Cell Signaling Technology

Product Pathways - Neuroscience

DCLK1/DCAMKL1 (D2U3L) XP® Rabbit mAb #62257

DCAM Kinase   DCAMK   DCAMKL1   DCL kinase  

No. Size Price
62257S 100 µl ( 10 western blots ) ¥3,580.00 现货查询 购买询价
62257 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 82 Rabbit IgG
IP 1:100
IHC-P 1:300

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin),

Specificity / Sensitivity

DCLK1/DCAMKL1 (D2U3L) XP® Rabbit mAb recognizes endogenous levels of total DCLK1/DCAMKL1 protein. The identity of the faint lower molecular weight bands is not known.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human DCLK1/DCAMKL1 protein.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded TT (left) and HeLa (right) cell pellets using DCLK1/DCAMKL1 (D2U3L) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded Apc (Min/+) mouse intestinal adenoma using DCLK1/DCAMKL1 (D2U3L) XP® Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines and tissues using DCLK1/DCAMKL1 (D2U3L) XP® Rabbit

mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). As expected, HeLa cells have extremely low expression of DCLK1/DCAMKL1.

IP

IP

Immunoprecipitation of DCLK1/ DCAMKL1 from TT cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb XP® Isotype Control #3900, and lane 3 is DCLK1/DCAMKL1 (D2U3L) XP® Rabbit mAb. Western blot analysis was performed using

DCLK1/DCAMKL1 (D2U3L) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using DCLK1/DCAMKL1 (D2U3L) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using DCLK1/DCAMKL1 (D2U3L) XP® Rabbit mAb.

Background

Doublecortin-like kinase 1 (DCLK1, DCAMKL1) is a serine/threonine kinase that belongs to the CaM kinase family and shares homology with the neuronal microtubule binding protein doublecortin. DCLK1 is thought to be involved in calcium signaling pathways controlling neuronal development in the embryonic brain (1,2). The kinase also functions in the mature nervous system and is highly expressed in regions of active neurogenesis in the neocortex and cerebellum (3,4). Research studies suggest that the DCLK1 kinase is highly expressed in subpopulations of cells within the colon and gastric epithelium and in the pancreas (5-8). The nature of these cell populations, whether normal, stem-like, or tumor-initiating, is unclear.

  1. Burgess, H.A. et al. (1999) J Neurosci Res 58, 567-75.
  2. Tanaka, T. et al. (2006) Cereb Cortex 16 Suppl 1, i69-73.
  3. Shin, E. et al. (2013) Nat Commun 4, 1440.
  4. Saaltink, D.J. et al. (2012) J Comp Neurol 520, 2805-23.
  5. May, R. et al. (2009) Stem Cells 27, 2571-9.
  6. May, R. et al. (2010) Am J Physiol Gastrointest Liver Physiol 299, G303-10.
  7. Saqui-Salces, M. et al. (2011) Histochem Cell Biol 136, 191-204.
  8. Qu, D. et al. (2014) Am J Physiol Gastrointest Liver Physiol 306, G404-11.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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