Cell Signaling Technology

Product Pathways - Metabolism

SignalSilence® Acetyl-CoA Carboxylase 1 siRNA I #6224

No. Size Price
6224S 300 µl ( 3 nmol ) ¥3,224.00 现货查询 购买询价
6224 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
TFN Human,

Species cross-reactivity is determined by western blot.

Applications Key: TFN=Transfection,

Description

SignalSilence® Acetyl-CoA Carboxylase 1 siRNA I from Cell Signaling Technology (CST) allows the researcher to specifically inhibit Acetyl-CoA Carboxylase 1 expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce protein expression by western analysis.

赛信通SignalSilence® Acetyl-CoA Carboxylase 1 siRNA I 可以通过RNA干扰技术特异性地抑制酰基辅酶A羟化酶1的表达(RNA干扰技术是通过往细胞内导入双链干扰RNA来选择性地抑制某 些基因的表达)。赛信通所有的SignalSilence® siRNA产品都经过了实验室试验的反复验证,并且通过Western实验来证实了基因表达的抑制效果。

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

寡核苷酸的合成由三苯甲基分析逐一检测碱基序列,以确保合适的耦合效率,然后通过亲和固相萃取纯化寡核苷酸。退火的双链RNA进一步通过质谱分析,以验证双链的确切组成。每批次产品 利用质谱检测,以最大程度地确保批次间的一致性。

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® Acetyl-CoA Carboxylase 1 siRNA I (+) or SignalSilence® Acetyl-CoA Carboxylase 1 siRNA II #6237 (+), using Acetyl-CoA Carboxylase 1 Antibody #4190 and α-Tubulin (11H10) Rabbit mAb #2125. The Acetyl-CoA Carboxylase 1 antibody confirms silencing of Acetyl-CoA Carboxylase 1 expression while the α-Tubulin (11H10) Rabbit mAb is used as a loading control.

Western blot检测HeLa细胞提取物,细胞用100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® Acetyl-CoA Carboxylase 1 siRNA I (+) 或 SignalSilence® Acetyl-CoA Carboxylase 1 siRNA II #6237 (+), 进行转染,检测抗体为Acetyl-CoA Carboxylase 1 Antibody #4190 (上) or α-Tubulin (11H10) Rabbit mAb兔单抗 #2125 (下)。 Acetyl-CoA Carboxylase 1 Antibody验证了酰基辅酶A羟化酶1基因的沉默情况,α-Tubulin (11H10) Rabbit mAb兔单抗是内参对照。

Directions for Use

CST recommends transfecting with 100 nM Acetyl-CoA Carboxylase 1 siRNA I 48 to 72 hours prior to cell lysis. For transfection procedure, follow protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use.

CST推荐对细胞用100 nM Acetyl-CoA Carboxylase 1 siRNA I转染48到72小时后再进行裂解。转染步骤参考转染试剂的厂家产品说明。如果在使用过程中有任何问题,请联系CST技术支持。

Background

Acetyl-CoA carboxylase (ACC) catalyzes the pivotal step of the fatty acid synthesis pathway. The 265 kDa ACCα (ACC1) is the predominant isoform found in liver, adipocytes, and mammary gland, while the 280 kDa ACCβ (ACC2) is the major isoform in skeletal muscle and heart (1). Phosphorylation by AMPK at Ser79 or by PKA at Ser1200 inhibits the enzymatic activity of ACC (2). ACC is a potential target of anti-obesity drugs (3,4).

乙酰辅酶A合成酶(ACC)用于催化脂肪酸合成途径中的关键步骤。分子量为265 kDa的ACCα,是肝脏,脂肪细胞和乳腺中ACC的主要形式,而在骨胳肌和心脏中,其主要形式为280 kDa的ACCβ (1)。ACC可以在Ser79被AMPK磷酸化,或者在Ser1200被PKA磷酸化,这些位点的磷酸化可以抑制ACC的酶活性(2)。ACC也是减肥药物的一个潜在靶点(3,4)。

  1. Castle, J.C. et al. (2009) PLoS One 4, e4369.
  2. Kreuz, S. et al. (2009) Diabetes Metab Res Rev 25, 577-86.
  3. Ha, J. et al. (1994) J Biol Chem 269, 22162-8.
  4. Abu-Elheiga, L. et al. (2001) Science 291, 2613-6.
  5. Levert, K.L. et al. (2002) J Biol Chem 277, 16347-50.

Application References

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Protocols

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

SignalSilence is a registered trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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