Cell Signaling Technology

Product Pathways - CD Markers

CD74 (D2X3O) Rabbit mAb (IF/Flow Cytometry Formulated) #61941

No. Size Price
61941S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
61941 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
F 1:50 Human, Endogenous Rabbit IgG
IF-IC 1:200

Species cross-reactivity is determined by western blot.

Applications Key: F=Flow Cytometry, IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

CD74 (D2X3O) Rabbit mAb (IF/Flow Cytometry Formulated) recognizes endogenous levels of total human CD74 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu284 of human CD74 protein.

IF-IC

IF-IC

Confocal immunofluorescent analysis of Daudi (positive, left) and K-562 (negative, right) cells using CD74 (D2X3O) Rabbit mAb (IF/Flow Cytometry Formulated) (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of K-562 (blue) and Daudi (green) cells using CD74 (D2X3O) Rabbit mAb (IF/Flow Cytometry Formulated). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.

Background

CD74, which is also known as the MHC Class II-associated invariant chain (Ii), is a type II transmembrane glycoprotein that plays a critical role in the antigen presentation process as a chaperone of MHC Class II proteins. It is expressed at high levels on B cells and to a lesser extent on numerous antigen presenting cell (APC) types including dendritic cells, Langerhans cells, monocytes, and macrophages as well as non-traditional APCs such as epithelial cells (1,2). CD74 was initially identified for its ability to regulate the folding and intracellular trafficking of newly synthesized MHC Class II molecules. Following expression, CD74 self-assembles as a trimer that serves as a scaffold for the assembly of MHC Class II molecules. Through this interaction, CD74 blocks the peptide binding cleft of MHC Class II molecules and prevents their premature association with endogenous polypeptides (3). Binding to CD74 also facilitates the translocation of MHC Class II molecules from the endoplasmic reticulum to the endocytic compartments during antigen presentation (4). In addition to its role as an MHC Class II chaperone, CD74 is also the receptor for macrophage migration-inhibitory factor (MIF). Binding to CD74 and its co-receptor, CD44, has been shown to induce the activation of the NFkB and ERK pathways to promote cell proliferation and survival signals (5,6). Recent studies have identified CXCR2 and CXCR4 as co-receptors for CD74 where MIF binding to CD74 complexes contributes to MIF-mediated monocyte chemotaxis and the induction of Akt signaling, respectively (7,8). Increased CD74 surface expression has been reported under inflammatory conditions and in certain types of cancer cells implying a potential role in tumorigenesis (9).

  1. Barrera, C.A. et al. (2005) J Histochem Cytochem 53, 1481-9.
  2. Lotteau, V. et al. (1990) Nature 348, 600-5.
  3. Stockinger, B. et al. (1989) Cell 56, 683-9.
  4. Bakke, O. and Dobberstein, B. (1990) Cell 63, 707-16.
  5. Shi, X. et al. (2006) Immunity 25, 595-606.
  6. Starlets, D. et al. (2006) Blood 107, 4807-16.
  7. Bernhagen, J. et al. (2007) Nat Med 13, 587-96.
  8. Schwartz, V. et al. (2009) FEBS Lett 583, 2749-57.
  9. Borghese, F. and Clanchy, F.I. (2011) Expert Opin Ther Targets 15, 237-51.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

DRAQ5 is a registered trademark of Biostatus Limited.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Alexa Fluor is a registered trademark of Life Technologies Corporation.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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