Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

β-Tubulin (9F3) Rabbit mAb (Biotinylated) #6181

TBB2   TUBB   TUBB2   TUBB5   Tubulin beta-2 chain   βtubulin  

No. Size Price
6181S 100 µl ( 10 western blots ) ¥3,986.00 现货查询 购买询价
6181 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey,Zebrafish,Bovine, Endogenous 55 Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,


Species predicted to react based on 100% sequence homology: Chicken,

Specificity / Sensitivity

β-Tubulin (9F3) Rabbit mAb (Biotinylated) detects endogenous levels of total β-tubulin protein, and does not cross-react with recombinant α-tubulin.

β-Tubulin (9F3) Rabbit mAb (Biotinylated)兔单抗检测内源性β-tubulin总蛋白,但是不能与重组的α-tubulin蛋白发生交叉反应。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human β-tubulin.



This Cell Signaling Technology antibody is conjugated to biotin under optimal conditions. The biotinylated antibody is expected to exhibit the same species cross-reactivity as the unconjugated β-Tubulin (9F3) Rabbit mAb #2128.

Cell Signaling Technology抗体以最佳条件下连接着biotin。该生物素抗体被期望与非连接的β-Tubulin (9F3) Rabbit mAb #2128拥有相同的物种交叉反应活性。

Western Blotting

Western Blotting

Western blot analysis of extracts from NIH/3T3, HeLa and PC-12 cells, using β-Tubulin (9F3) Rabbit mAb (Biotinylated) and detected using Streptavidin-HRP #3999.

使用β-Tubulin (9F3) Rabbit mAb (Biotinylated),并且使用Streptavidin-HRP #3999检测,免疫印迹(Western Blot)分析NIH/3T3、HeLa和PC-12细胞中β-Tubulin蛋白。


The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. γ-tubulin is required to nucleate polymerization of tubulin subunits to form microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from competitive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins (1).


  1. Westermann, S. and Weber, K. (2003) Nat Rev Mol Cell Biol 4, 938-47.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

U.S. Patent No. 5,675,063.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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