Cell Signaling Technology

Product Pathways - Metabolism

SAT1 (D1T7M) Rabbit mAb #61586

SAT   SAT1  

No. Size Price
61586S 100 µl ( 10 western blots ) ¥3,250.00 现货查询 购买询价 防伪查询
61586 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 18 Rabbit IgG
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,


Species predicted to react based on 100% sequence homology: Mouse,

Specificity / Sensitivity

SAT1 (D1T7M) Rabbit mAb recognizes endogenous levels of total SAT1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Trp132 of human SAT1 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from SK-MEL-28 cells untreated (-) or treated with N1, N11-diethylnorspermine (DENSPM) (10 μM, 24 hr; +), using SAT1 (D1T7M) Rabbit mAb.


Spermidine/spermine N1-acetyltransferase 1 (SAT1) is a key enzyme in polyamine metabolism. It acts by acetylating spermidine and spermine using acetyl-coenzyme A (CoA), which alters their charge and facilitates their secretion (1,2). SAT1 activity is tightly controlled in cells, but increases quickly by excess polyamines. Its activity can also be induced by a number of other stimuli, such as oxidative stress, heat shock, insulin-like growth factor-I, and cytotoxins (2-4). Research studies have found that SAT1 is overexpressed in glioblastoma multiforme (GBM), which suggest that SAT1 may be a potential therapeutic target for GBM (5).

  1. Casero, R.A. and Pegg, A.E. (1993) FASEB J 7, 653-61.
  2. Pegg, A.E. (2008) Am J Physiol Endocrinol Metab 294, E995-1010.
  3. Butcher, N.J. et al. (2007) J Biol Chem 282, 28530-9.
  4. Perez-Leal, O. et al. (2012) Mol Cell Biol 32, 1453-67.
  5. Brett-Morris, A. et al. (2014) Cancer Res 74, 6925-34.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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