Cell Signaling Technology

Product Pathways - Metabolism

PBEF/NAMPT (D1K6D) Rabbit mAb #61122

NAMPT   PBEF   Visfatin  

No. Size Price
61122S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
61122 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 52 Rabbit IgG
IP 1:200

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

PBEF/NAMPT (D1K6D) Rabbit mAb recognizes endogenous levels of total PBEF/NAMPT protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly122 of human PBEF/NAMPT protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa, HT-1080, and DLD-1 cells using PBEF/NAMPT (D1K6D) Rabbit mAb.

Background

Nicotinamide phosphoribosyltransferase (NAMPT; also known as Pre-B cell-enhancing factor PBEF) catalyzes the synthesis of nicotinamide mononucleotide (NMN) from nicotinamide and 5-phosphoribosylpyrophosphate (PRPP), the rate-limiting step in the NAD biosynthesis pathway starting from nicotinamide (1,2). NAD biosynthesis mediated by NAMPT plays a critical role in glucose-stimulated insulin secretion in pancreatic beta cells (3). Both NAMPT inhibitors and activators have been sought for clinical applications (4,5). NAMPT has intra- and extracellular forms (iNAMPT and eNAMPT), and deacetylation of iNAMPT by SIRT1 promotes eNAMPT secretion through a nonclassical secretory pathway (3,6). eNAMPT, independent of its enzymatic activity, can induce epithelial-to-mesenchymal transition in mammary epithelial cells and promote monocyte differentiation into a tumor-supporting M2 macrophage (7,8).

  1. Imai, S. (2009) Curr Pharm Des 15, 20-8.
  2. Samal, B. et al. (1994) Mol Cell Biol 14, 1431-7.
  3. Revollo, J.R. et al. (2007) Cell Metab 6, 363-75.
  4. Montecucco, F. et al. (2013) Curr Drug Targets 14, 637-43.
  5. Wang, G. et al. (2014) Cell 158, 1324-34.
  6. Yoon, M.J. et al. (2015) Cell Metab 21, 706-17.
  7. Soncini, D. et al. (2014) J Biol Chem 289, 34189-204.
  8. Audrito, V. et al. (2015) Blood 125, 111-23.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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