Cell Signaling Technology

Product Pathways - Vesicle Trafficking

Importin β1 (E1F1G) Rabbit mAb #60769

No. Size Price
60769S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
60769 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 97 Rabbit IgG
IP 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

Importin β1 (E1F1G) Rabbit mAb recognizes endogenous levels of total importin β1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asn486 of human importin β1 protein.

IP

IP

Immunoprecipitation of Importin β1 from HeLa cell extracts. Lane 1 is 10% input, lane 2 is Normal Rabbit IgG #3900, and lane 3 is Importin β1 (E1F1G) Rabbit mAb. Western blot analysis was performed using Importin β1 (E1F1G) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Importin β1 (E1F1G) Rabbit mAb.

Background

Importins belong to the karyopherin family of nuclear transport proteins (1) and are divided into two subgroups: importin α and importin β. Importins mainly function in nuclear protein import and export (2,3). Importin β1 (also known as karyopherin β1, Kpnβ1, Kpnb1, or p97) plays a key role in the nuclear import process (1-3). Nuclear import via importin β1 association with adaptor importin α (also known as karyopherin α, or Kpnα) is an essential component of the classical nuclear localization signal (NLS) pathway (4). Importin α directly recognizes the NLS present in the cargo target, prompting complex formation with importin β1. The cargo:importin α:importin β1 complex is transported across the nuclear pore complex (NPC) into the nucleus, where it is dissociated by the binding of RanGTP (1-4). Nuclear import directly via importin β1 can also occur by importin β1 recognition of the cargo protein, bypassing importin α involvement. In both cases, the importin β1/target protein interaction is mediated through the binding of importin β1 HEAT repeats with the target protein sequences (either the cargo protein itself or importin α) (5).

  1. Chook, Y.M. and Blobel, G. (2001) Curr Opin Struct Biol 11, 703-15.
  2. Pemberton, L.F. and Paschal, B.M. (2005) Traffic 6, 187-98.
  3. Moroianu, J. (1998) J Cell Biochem 70, 231-9.
  4. Lange, A. et al. (2007) J Biol Chem 282, 5101-5.
  5. Marfori, M. et al. (2011) Biochim Biophys Acta 1813, 1562-77.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Tween is a registered trademark of ICI Americas, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0

该产品暂无评论!

我要参与评论 :

如要参与评论请先登录网站

还没有网站账户?去注册一下吧

Products

 

Applications