Product Pathways - Adhesion
p130 Cas (E1L9H) Rabbit mAb (PE Conjugate) #59987
|59987S||100 µl ( 50 tests )||￥4,060.00 现货查询||购买询价|
|59987||carrier free & custom formulation / quantity||email request|
Species cross-reactivity is determined by western blot.
Applications Key: F=Flow Cytometry,
Specificity / Sensitivity
p130 Cas (E1L9H) Rabbit mAb (PE Conjugate) recognizes endogenous levels of total p130 Cas protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu116 in the SH3 domain of human p130 Cas protein.
This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometric analysis in human cells. This antibody is expected to exhibit the same species cross-reactivity as the unconjugated p130 Cas (E1L9H) Rabbit mAb #13846.
p130 Cas (Crk-associated substrate) is a docking protein containing multiple protein-protein interaction domains. The amino-terminal SH3 domain may function as a molecular switch regulating CAS tyrosine phosphorylation, as it interacts with focal adhesion kinase (FAK) (1) and the FAK-related kinase PYK2 (2), as well as the tyrosine phosphatases PTP-1B (3) and PTP-PEST (4). The carboxy-terminal Src binding domain (SBD) contains a proline-rich motif that mediates interaction with the SH3 domains of Src-family kinases (SFKs) and a tyrosine phosphorylation site (Tyr668 and/or Tyr670) that can promote interaction with the SH2 domain of SFKs (5). The p130 Cas central substrate domain, the major region of tyrosine phosphorylation, is characterized by 15 tyrosines present in Tyr-X-X-Pro (YXXP) motifs, including Tyr165, 249, and 410. When phosphorylated, most YXXP motifs are able to serve as docking sites for proteins with SH2 or PTB domains including adaptors, C-Crk, Nck, and inositol 5'-phosphatase 2 (SHIP2) (6). The tyrosine phosphorylation of p130 Cas has been implicated as a key signaling step in integrin control of normal cellular behaviors including motility, proliferation, and survival. Aberrant Cas tyrosine phosphorylation may contribute to cell transformation by certain oncoproteins (5).
- Polte, T.R. and Hanks, S.K. (1997) J Biol Chem 272, 5501-9.
- Astier, A. et al. (1997) J Biol Chem 272, 228-32.
- Liu, F. et al. (1996) J Biol Chem 271, 31290-5.
- Garton, A.J. et al. (1997) Oncogene 15, 877-85.
- Ruest, P.J. et al. (2001) Mol Cell Biol 21, 7641-52.
- Bouton, A.H. et al. (2001) Oncogene 20, 6448-58.
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For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
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