Cell Signaling Technology

Product Pathways - DNA Damage

TDP1 (D8D1B) Rabbit mAb #59710

No. Size Price
59710S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
59710 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 70 Rabbit IgG
IF-IC 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

TDP1 (D8D1B) Rabbit mAb recognizes endogenous levels of total TDP1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro587 of human TDP1 protein.

IF-IC

IF-IC

Confocal immunofluorescent analysis of MDA-MB-231 (left, high expressing), SK-MEL-28 (center, moderately expressing), and MRC-5 (right, lower expressing) cells using TDP1 (D8D1B) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red).

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using TDP1 (D8D1B) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

Background

Topoisomerases are ubiquitous, conserved enzymes that remove DNA supercoils resulting from processes such as chromosome segregation, DNA replication, transcription, and repair (1). Topoisomerase inhibitors such as camptothecin and etoposide trap the enzyme as a DNA-bound intermediate, and these drugs are used to treat multiple human cancers (1,2). Tyrosyl-DNA-phosphodiesterases TDP1 and TDP2 function in the base excision repair (BER) and nonhomologous end joining (NHEJ) DNA repair pathways, respectively, and function in part in the repair of stalled topoisomerase-DNA complexes (3). Research has shown that inhibitors of tyrosyl-DNA-phosphodiesterases may act synergistically with topoisomerase inhibitors, allowing the potential for a more robust treatment of cancer (4,5). In small cell lung cancer, research suggests that TDP1 and topoisomerase 1 levels can predict sensitivity to topoisomerase 1 inhibitors (6).

  1. Pommier, Y. (2013) ACS Chem Biol 8, 82-95.
  2. Saijo, N. (2000) Ann N Y Acad Sci 922, 92-9.
  3. Pommier, Y. et al. (2014) DNA Repair (Amst) 19, 114-29.
  4. Nguyen, T.X. et al. (2012) J Med Chem 55, 4457-78.
  5. Huang, S.N. et al. (2011) Expert Opin Ther Pat 21, 1285-92.
  6. Meisenberg, C. et al. (2014) J Cancer Sci Ther 6, 258-67.

Application References

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Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Tween is a registered trademark of ICI Americas, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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