Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

TDRD3 (D3O2G) Rabbit mAb #5942

No. Size Price
5942S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
5942 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 83 Rabbit IgG
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

TDRD3 (D3O2G) Rabbit mAb recognizes endogenous levels of total TDRD3 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human TDRD3 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using TDRD3 (D3O2G) Rabbit mAb.

IP

IP

Immunoprecipitation of TDRD3 from SH-SY5Y cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is TDRD3 (D3O2G) Rabbit mAb. Western blot analysis was performed using TDRD3 (D3O2G) Rabbit mAb.

Background

Tudor domain-containing protein 3 (TDRD3) contains a tudor domain through which it binds to asymmetric di-methyl histone H3 (Arg17) and asymmetric di-methyl histone H4 (Arg3). Both of these histone marks are associated with transcription activation (1,2). TDRD3 is targeted to estrogen responsive genes where it performs as a coactivator (1). TDRD3 acts as a scaffolding protein to recruit Topoisomerase III B (TOP3B) to target genes to reduce transcription-generated R loops by relaxing negatively supercoiled DNA thereby reducing genomic instability (3). In addition to the nucleus, TDRD3 also resides in the cytoplasm where it associates with actively translating polyribosomes and accumulates into stress granules upon exposure to stress, implicating its role in post-transcriptional regulation of RNA (2,4).

  1. Yang, Y. et al. (2010) Mol Cell 40, 1016-23.
  2. Goulet, I. et al. (2008) Hum Mol Genet 17, 3055-74.
  3. Yang, Y. et al. (2014) Mol Cell 53, 484-97.
  4. Linder, B. et al. (2008) Hum Mol Genet 17, 3236-46.

Application References

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Protocols

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For Research Use Only. Not For Use In Diagnostic Procedures.

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