Cell Signaling Technology

Product Pathways - Phosphatases

Okadaic Acid #5934

PP1 inhibitor   PP2A inhibitor   reversible phosphorylation of proteins  

Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype

Species cross-reactivity is determined by western blot.

Applications Key:

Molecular Characterization

C44H68O13

Purity

>98%

Structure

Structure

Chemical structure of okadaic acid.冈田酸的化学结构。

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, untreated (-) or treated with Okadaic Acid (1 µM, 1 hr; +), using Phospho-LATS1 (Thr1079) (D57D3) Rabbit mAb #8654 (upper) or LATS1 (C66B5) Rabbit mAb #3477 (lower).Western blot分析HeLa细胞提取物,未处理(-)或用冈田酸(1 µM, 1小时; +)处理,使用的抗体是:Phospho-LATS1 (Thr1079) (D57D3) Rabbit mAb #8654(上图)、LATS1 (C66B5) Rabbit mAb #3477(下图)。

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, untreated or treated with Okadaic Acid (1 hr) at the indicated concentrations, using Phospho-Threonine Antibody (P-Thr-Polyclonal) #9381 (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).Western blot分析HeLa细胞提取物,未处理或用冈田酸(1小时)按照方案设计好的浓度处理,使用的抗体是:Phospho-Threonine Antibody (P-Thr-Polyclonal) #9381(上图)、β-Actin (D6A8) Rabbit mAb #8457(下图)。

Directions for Use

Okadaic Acid is supplied as a lyophilized powder. For a 1 mM stock, reconstitute the 25 µg in 31.1 µl DMSO. Working concentrations and length of treatments vary depending on the desired effect, but it is typically used at 10-1000 nM for 15-60 min. Soluble in DMSO or ethanol.

提供的冈田酸为冻干粉。对于1 mM浓度贮存液,在25 µg冻干粉中加入31.1 µl DMSO。工作浓度和处理时间根据所需的预期效果来确定,一般是10-1000 nM,处理15-60分钟。能溶于DMSO或乙醇。

Background

Okadaic acid is an inhibitor of the serine/theorine protein phosphatases PP1 (IC50 = 15-20 nM) and PP2A (IC50 = 0.1 nM), exhibiting much greater activity towards the latter. PP2A is completely inhibited at 1-2 nM, compared to greater than 1 µM for PP1 (1). This is unlike the protein phosphatase inhibitor Calyculin A, which potently inhibits both PP1 (IC50 = 2 nM) and PP2A (IC50 = 0.5-1 nM) (2). Okadaic acid does not effectively inhibit PP2C (3), acid and alkaline phosphatases, nor phosphotyrosine protein phosphatases (2,3). It has shown to inhibit PP2B, but at much higher concentrations than with PP1 and PP2A (3). Okadaic acid has also been described as a non-TPA type potent tumor promoter (4). These tumor-promoting properties and its ability to significantly increase protein phosphorylation make okadaic acid a very useful tool for studying cellular processes that are regulated by reversible phosphorylation of proteins (5).

冈田酸是一种丝氨酸/苏氨酸蛋白磷酸酶PP1 (IC50 = 15-20 nM)和PP2A (IC50 = 0.1 nM)的抑制剂,并且其对后者显示出更高的活性。PP2A在1-2 nM就能被完全抑制,而PP1则要大于1 µM(1)。它与蛋白磷酸酶抑制剂花萼海绵诱癌素A不一样,花萼海绵诱癌素A对PP1 (IC50 = 2 nM)和PP2A (IC50 = 0.5-1 nM)都能有效抑制(2)。冈田酸不能有效抑制PP2C(3),酸性和碱性磷酸酶以及磷酸酪氨酸蛋白磷酸酶(2,3)。它能抑制PP2B,但是所需浓度要大大高于抑制PP1和PP2A所用浓度(3)。冈田酸还是一种非TPA型的有效的肿瘤促进剂(4)。这些肿瘤促进的性质和能显著增加蛋白质磷酸化的能力使得冈田酸是一种研究细胞进程(由可逆的蛋白质的磷酸化调节)的有用工具(5)。

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0

该产品暂无评论!

我要参与评论 :

如要参与评论请先登录网站

还没有网站账户?去注册一下吧

Products

 

Applications