Cell Signaling Technology

Product Pathways - DNA Damage

Doxorubicin #5927

Adriamycin   DNA synthesis inhibitor   DNA topoisomerase inhibitor   p53   RNA synthesis inhibitor  

Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype

Species cross-reactivity is determined by western blot.

Applications Key:

Molecular Characterization

C27H29NO11 • HCl





Chemical structure of doxorubicin.多柔比星的化学结构。

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, serum-starved overnight and untreated or treated with Doxorubicin (5 μM) for the indicated times, using Phospho-p53 (Ser15) Antibody #9284, p53 Antibody #9282, Cleaved Caspase-3 (Asp175) Antibody #9661, or PARP Antibody #9542.Western blot分析HeLa细胞提取物,血清饥饿过夜,未处理或用多柔比星(5 μM)按照方案中设计好的时间处理,使用的抗体是:Phospho-p53 (Ser15) Antibody #9284、p53 Antibody #9282、Cleaved Caspase-3 (Asp175) Antibody #9661、PARP Antibody #9542。

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, untreated, treated with Doxorubicin (0.5 µM, 24 hr), or treated with Trichostatin A (TSA) #9950 (400 nM, 24 hr) and Doxorubicin (0.5 µM, 24 hr), using Acetyl-p53 (Lys382) Antibody #2525 (upper) or p53 (1C12) Mouse mAb #2524 (lower).Western blot分析HeLa细胞提取物,未处理,用多柔比星(0.5 µM, 24小时)处理或用Trichostatin A (TSA) #9950 (400 nM, 24小时)和多柔比星(0.5 µM, 24小时)处理,使用的抗体是:Acetyl-p53 (Lys382) Antibody #2525(上图)、p53 (1C12) Mouse mAb #2524(下图)。

Directions for Use

Doxorubicin is supplied as a lyophilized powder. For a 10 mM stock, reconstitute the 5 mg in 860 µl DMSO. Working concentrations and length of treatments vary depending on the desired effect, but it is typically used at 0.1-5 µM for 12-24 hours. Soluble in DMSO at 100 mg/ml; very poorly soluble in ethanol; soluble in water at 10 mg/ml with slight warming.

提供的多柔比星为冻干粉。对于10 mM浓度贮存液,在5mg冻干粉中加入860 µl DMSO。工作浓度和处理时间根据所需的预期效果来确定,一般是0.1-5 µM,处理12-24小时。能溶于DMSO(100 mg/ml)和微热的水(10 mg/ml),难溶于乙醇。


Doxorubicin, an anthracycline antibiotic, inhibits DNA and RNA synthesis in mammalian cells and has been shown to be a very effective anti-tumor agent (1,2). Doxorubicin binds to nucleic acids by intercalating the DNA double helix and stabilizing topoisomerase II cleavage complexes, leading to DNA strand breaks at specific doxorubicin-induced sites (3). Doxorubin has been shown to inhibit DNA synthesis in a dose-dependent manner in MCF7 cells, which corresponds closely with growth inhibition (4). Researchers have also demonstrated that doxorubicin effectively inhibits human DNA topoisomerase I (5).


Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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