Cell Signaling Technology

Product Pathways - DNA Damage

Doxorubicin #5927

Adriamycin   DNA synthesis inhibitor   DNA topoisomerase inhibitor   p53   RNA synthesis inhibitor  

Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype

Species cross-reactivity is determined by western blot.

Applications Key:

Molecular Characterization

C27H29NO11 • HCl

Purity

>99%

Structure

Structure

Chemical structure of doxorubicin.多柔比星的化学结构。

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, serum-starved overnight and untreated or treated with Doxorubicin (5 μM) for the indicated times, using Phospho-p53 (Ser15) Antibody #9284, p53 Antibody #9282, Cleaved Caspase-3 (Asp175) Antibody #9661, or PARP Antibody #9542.Western blot分析HeLa细胞提取物,血清饥饿过夜,未处理或用多柔比星(5 μM)按照方案中设计好的时间处理,使用的抗体是:Phospho-p53 (Ser15) Antibody #9284、p53 Antibody #9282、Cleaved Caspase-3 (Asp175) Antibody #9661、PARP Antibody #9542。

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, untreated, treated with Doxorubicin (0.5 µM, 24 hr), or treated with Trichostatin A (TSA) #9950 (400 nM, 24 hr) and Doxorubicin (0.5 µM, 24 hr), using Acetyl-p53 (Lys382) Antibody #2525 (upper) or p53 (1C12) Mouse mAb #2524 (lower).Western blot分析HeLa细胞提取物,未处理,用多柔比星(0.5 µM, 24小时)处理或用Trichostatin A (TSA) #9950 (400 nM, 24小时)和多柔比星(0.5 µM, 24小时)处理,使用的抗体是:Acetyl-p53 (Lys382) Antibody #2525(上图)、p53 (1C12) Mouse mAb #2524(下图)。

Directions for Use

Doxorubicin is supplied as a lyophilized powder. For a 10 mM stock, reconstitute the 5 mg in 860 µl DMSO. Working concentrations and length of treatments vary depending on the desired effect, but it is typically used at 0.1-5 µM for 12-24 hours. Soluble in DMSO at 100 mg/ml; very poorly soluble in ethanol; soluble in water at 10 mg/ml with slight warming.

提供的多柔比星为冻干粉。对于10 mM浓度贮存液,在5mg冻干粉中加入860 µl DMSO。工作浓度和处理时间根据所需的预期效果来确定,一般是0.1-5 µM,处理12-24小时。能溶于DMSO(100 mg/ml)和微热的水(10 mg/ml),难溶于乙醇。

Background

Doxorubicin, an anthracycline antibiotic, inhibits DNA and RNA synthesis in mammalian cells and has been shown to be a very effective anti-tumor agent (1,2). Doxorubicin binds to nucleic acids by intercalating the DNA double helix and stabilizing topoisomerase II cleavage complexes, leading to DNA strand breaks at specific doxorubicin-induced sites (3). Doxorubin has been shown to inhibit DNA synthesis in a dose-dependent manner in MCF7 cells, which corresponds closely with growth inhibition (4). Researchers have also demonstrated that doxorubicin effectively inhibits human DNA topoisomerase I (5).

多柔比星,一种蒽环类抗生素,能抑制哺乳动物中DNA和RNA的合成,也是一种非常有效的抗肿瘤药物(1,2)。多柔比星通过嵌入DNA双螺旋和稳定拓扑异构酶II裂解复合物来结合到核酸,导致DNA在特异的多柔比星诱导的位点发生丝条断裂(3)。在人乳腺癌细胞(MCF7)中,多柔比星能通过一种剂量依赖的方式抑制DNA合成,与生长抑制相符合(4)。研究者还发现多柔比星能够有效抑制人DNA拓扑异构酶I(5)。

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0

该产品暂无评论!

我要参与评论 :

如要参与评论请先登录网站

还没有网站账户?去注册一下吧

Products

 

Applications