Cell Signaling Technology

Product Pathways - Innate Immunity

IFITM3 (D8E8G) XP® Rabbit mAb #59212

No. Size Price
59212S 100 µl ( 10 western blots ) ¥3,580.00 现货查询 购买询价
59212 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 15 Rabbit IgG
IP 1:200
IHC-P 1:250

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin),

Specificity / Sensitivity

IFITM3 (D8E8G) XP® Rabbit mAb recognizes endogenous levels of total IFITM3 protein. This antibody does not cross-react with IFITM1 or IFITM2 proteins.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val5 of human IFITM3 protein.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using IFITM3 (D8E8G) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded HeLa (left) and RPMI 8226 (right) cell pellets using IFITM3 (D8E8G) XP® Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells untransfected (-), or transfected with a construct expressing Myc-tagged full-length human IFITM3 (hIFITM3-Myc; +), using IFITM3 (D8E8G) XP® Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using IFITM3 (D8E8G) XP® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded normal human lymph node using IFITM3 (D8E8G) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded normal human spleen using IFITM3 (D8E8G) XP® Rabbit mAb.

Background

Interferon-induced transmembrane protein (IFITM) family members are composed of short amino- and carboxy-termini, two transmembrane domains, and a cytoplasmic domain (1). There are four family members in humans: IFITM1, IFITM2, IFITM3, and IFITM5 (2,3). Mice have two additional family members, IFITM6 and IFITM7 (2,3). Basal expression of IFITM proteins is observed in some cells and expression can also be induced by type I and type II interferons (4-6). The primary function of IFITM family proteins appears to be viral restriction, as IFITM proteins inhibit cytosolic entry of viruses by preventing fusion of viral and host membranes (7,8). The mechanism by which IFITM proteins inhibit fusion is unclear. Although IFITM proteins are present on both the plasma membrane and intracellular membranes, they most effectively restrict viral fusion in late endosomes and lysosomes (8,9). In addition, different family members exhibit specific viral preferences (9). For example, IFITM3 is most effective at restricting influenza A infection, while IFITM1 is more successful in controlling filoviruses and SARS (9,10).

  1. Diamond, M.S. and Farzan, M. (2013) Nat Rev Immunol 13, 46-57.
  2. Lange, U.C. et al. (2003) BMC Dev Biol 3, 1.
  3. Hickford, D. et al. (2012) BMC Genomics 13, 155.
  4. Reid, L.E. et al. (1989) Proc Natl Acad Sci U S A 86, 840-4.
  5. Lewin, A.R. et al. (1991) Eur J Biochem 199, 417-23.
  6. Friedman, R.L. et al. (1984) Cell 38, 745-55.
  7. Brass, A.L. et al. (2009) Cell 139, 1243-54.
  8. Feeley, E.M. et al. (2011) PLoS Pathog 7, e1002337.
  9. Huang, I.C. et al. (2011) PLoS Pathog 7, e1001258.
  10. Everitt, A.R. et al. (2012) Nature 484, 519-23.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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