Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

CENP-F (D6X4L) Rabbit mAb #58982

No. Size Price
58982S 100 µl ( 10 western blots ) ¥3,250.00 现货查询 购买询价 防伪查询
58982 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 350 Rabbit IgG
F 1:50
IF-IC 1:800

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, F=Flow Cytometry, IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

CENP-F (D6X4L) Rabbit mAb recognizes endogenous levels of total CENP-F protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala1794 of human CENP-F protein.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Jurkat cells using CENP-F (D6X4L) Rabbit mAb and Propidium Iodide (PI)/RNase Staining Solution #4087 to measure DNA content. Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.

Western Blotting

Western Blotting

Western blot analysis of HT-29 cells, untreated (-) or synchronized in mitosis by treating with thymidine (2 mM, 16 hr) followed by Nocodazole #2190 (10 nM, 24 hr; +), using CENP-F (D6X4L) Rabbit mAb (upper) or α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower).



Confocal immunofluorescent analysis of HT-29 cells using CENP-F (D6X4L) Rabbit mAb (green) and a Cyclin B1 antibody (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).



Confocal immunofluorescent analysis of HT-29 cells at various stages of mitosis using CENP-F (D6X4L) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).


CENP-F (mitosin), is a kinetochore-associated protein whose expression and localization to chromatin is regulated in a cell cycle-dependent manner, with its highest expression in G2/M phases (1, 2). CENP-F is required for appropriate localization of other kinetocore-associated proteins, including CENP-E. CENP-F regulates kinetocore function and maintenance of the mitotic spindle checkpoint. Farnesylation of CENP-F is required for its localization and function (3). CENP-F also interacts with the mitochondrial protein, miro, to direct the distribution of mitochondria to daughter cells as they exit mitosis (4). Researchers have shown that CENP-F drives prostate tumor growth synergistically with FOXM1 in human and mouse (5), and that the gene for CENP-F is among those frequently amplified in hepatocellular, head and neck, and esophageal carcinomas (6-8). CENP-F expression has also been shown in research studies to be associated with poor prognosis in breast cancer (9).

  1. Rattner, J.B. et al. (1993) Cell Motil Cytoskeleton 26, 214-26.
  2. Liao, H. et al. (1995) J Cell Biol 130, 507-18.
  3. Ma, L. et al. (2006) J Biomed Sci 13, 205-13.
  4. Kanfer, G. et al. (2015) Nat Commun 6, 8015.
  5. Aytes, A. et al. (2014) Cancer Cell 25, 638-51.
  6. Kim, H.E. et al. (2012) PLoS One 7, e43223.
  7. Pimkhaokham, A. et al. (2000) Jpn J Cancer Res 91, 1126-33.
  8. de la Guardia, C. et al. (2001) Head Neck 23, 104-12.
  9. O'Brien, S.L. et al. (2007) Int J Cancer 120, 1434-43.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

DRAQ5 is a registered trademark of Biostatus Limited.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

Alexa Fluor is a registered trademark of Life Technologies Corporation.

Tween is a registered trademark of ICI Americas, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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