Product Pathways - Cell Cycle / Checkpoint
CENP-F (D6X4L) Rabbit mAb #58982
|58982S||100 µl ( 10 western blots )||￥3,250.00||现货查询 购买询价 防伪查询|
|58982||carrier free & custom formulation / quantity||email request|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, F=Flow Cytometry, IF-IC=Immunofluorescence (Immunocytochemistry),
Specificity / Sensitivity
CENP-F (D6X4L) Rabbit mAb recognizes endogenous levels of total CENP-F protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala1794 of human CENP-F protein.
Flow cytometric analysis of Jurkat cells using CENP-F (D6X4L) Rabbit mAb and Propidium Iodide (PI)/RNase Staining Solution #4087 to measure DNA content. Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
Western blot analysis of HT-29 cells, untreated (-) or synchronized in mitosis by treating with thymidine (2 mM, 16 hr) followed by Nocodazole #2190 (10 nM, 24 hr; +), using CENP-F (D6X4L) Rabbit mAb (upper) or α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower).
Confocal immunofluorescent analysis of HT-29 cells using CENP-F (D6X4L) Rabbit mAb (green) and a Cyclin B1 antibody (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
CENP-F (mitosin), is a kinetochore-associated protein whose expression and localization to chromatin is regulated in a cell cycle-dependent manner, with its highest expression in G2/M phases (1, 2). CENP-F is required for appropriate localization of other kinetocore-associated proteins, including CENP-E. CENP-F regulates kinetocore function and maintenance of the mitotic spindle checkpoint. Farnesylation of CENP-F is required for its localization and function (3). CENP-F also interacts with the mitochondrial protein, miro, to direct the distribution of mitochondria to daughter cells as they exit mitosis (4). Researchers have shown that CENP-F drives prostate tumor growth synergistically with FOXM1 in human and mouse (5), and that the gene for CENP-F is among those frequently amplified in hepatocellular, head and neck, and esophageal carcinomas (6-8). CENP-F expression has also been shown in research studies to be associated with poor prognosis in breast cancer (9).
- Rattner, J.B. et al. (1993) Cell Motil Cytoskeleton 26, 214-26.
- Liao, H. et al. (1995) J Cell Biol 130, 507-18.
- Ma, L. et al. (2006) J Biomed Sci 13, 205-13.
- Kanfer, G. et al. (2015) Nat Commun 6, 8015.
- Aytes, A. et al. (2014) Cancer Cell 25, 638-51.
- Kim, H.E. et al. (2012) PLoS One 7, e43223.
- Pimkhaokham, A. et al. (2000) Jpn J Cancer Res 91, 1126-33.
- de la Guardia, C. et al. (2001) Head Neck 23, 104-12.
- O'Brien, S.L. et al. (2007) Int J Cancer 120, 1434-43.
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
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For Research Use Only. Not For Use In Diagnostic Procedures.
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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
Alexa Fluor is a registered trademark of Life Technologies Corporation.
Tween is a registered trademark of ICI Americas, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
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