Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

CENP-F (D6X4L) Rabbit mAb #58982

No. Size Price
58982S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
58982 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 350 Rabbit IgG
F 1:50
IF-IC 1:800

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, F=Flow Cytometry, IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

CENP-F (D6X4L) Rabbit mAb recognizes endogenous levels of total CENP-F protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala1794 of human CENP-F protein.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Jurkat cells using CENP-F (D6X4L) Rabbit mAb and Propidium Iodide (PI)/RNase Staining Solution #4087 to measure DNA content. Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.

Western Blotting

Western Blotting

Western blot analysis of HT-29 cells, untreated (-) or synchronized in mitosis by treating with thymidine (2 mM, 16 hr) followed by Nocodazole #2190 (10 nM, 24 hr; +), using CENP-F (D6X4L) Rabbit mAb (upper) or α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower).

IF-IC

IF-IC

Confocal immunofluorescent analysis of HT-29 cells using CENP-F (D6X4L) Rabbit mAb (green) and a Cyclin B1 antibody (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

IF-IC

IF-IC

Confocal immunofluorescent analysis of HT-29 cells at various stages of mitosis using CENP-F (D6X4L) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Background

CENP-F (mitosin), is a kinetochore-associated protein whose expression and localization to chromatin is regulated in a cell cycle-dependent manner, with its highest expression in G2/M phases (1, 2). CENP-F is required for appropriate localization of other kinetocore-associated proteins, including CENP-E. CENP-F regulates kinetocore function and maintenance of the mitotic spindle checkpoint. Farnesylation of CENP-F is required for its localization and function (3). CENP-F also interacts with the mitochondrial protein, miro, to direct the distribution of mitochondria to daughter cells as they exit mitosis (4). Researchers have shown that CENP-F drives prostate tumor growth synergistically with FOXM1 in human and mouse (5), and that the gene for CENP-F is among those frequently amplified in hepatocellular, head and neck, and esophageal carcinomas (6-8). CENP-F expression has also been shown in research studies to be associated with poor prognosis in breast cancer (9).

  1. Rattner, J.B. et al. (1993) Cell Motil Cytoskeleton 26, 214-26.
  2. Liao, H. et al. (1995) J Cell Biol 130, 507-18.
  3. Ma, L. et al. (2006) J Biomed Sci 13, 205-13.
  4. Kanfer, G. et al. (2015) Nat Commun 6, 8015.
  5. Aytes, A. et al. (2014) Cancer Cell 25, 638-51.
  6. Kim, H.E. et al. (2012) PLoS One 7, e43223.
  7. Pimkhaokham, A. et al. (2000) Jpn J Cancer Res 91, 1126-33.
  8. de la Guardia, C. et al. (2001) Head Neck 23, 104-12.
  9. O'Brien, S.L. et al. (2007) Int J Cancer 120, 1434-43.

Application References

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Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

DRAQ5 is a registered trademark of Biostatus Limited.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

Alexa Fluor is a registered trademark of Life Technologies Corporation.

Tween is a registered trademark of ICI Americas, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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