Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

TCPTP (TC45) (D7T7D) Rabbit mAb #58935

TC45  

No. Size Price
58935S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
58935 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Hamster,Monkey, Endogenous 45 Rabbit IgG
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Homology

Species predicted to react based on 100% sequence homology: Bovine, Dog, Pig,

Specificity / Sensitivity

TCPTP (TC45) (D7T7D) Rabbit mAb recognizes endogenous levels of total TCPTP (TC45) protein. This antibody does not recognize the 48 kDa (TC48) isoform.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro382 of human TCPTP (TC45) protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using TCPTP (TC45) (D7T7D) Rabbit mAb.

IP

IP

Immunoprecipitation of TCPTP (TC45) from Rec-1 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is TCPTP (TC45) (D7T7D) Rabbit mAb. Western blot analysis was performed using TCPTP (TC45) (D7T7D) Rabbit mAb.

Background

T cell protein tyrosine phosphatase (TCPTP, PTPN2, PTN2) is a non-receptor protein tyrosine phosphatase (PTP) that regulates signal transduction pathways by catalyzing the dephosphorylation of tyrosine residues (1). Two described TCPTP splice variants include a 48 kDa isoform (TC48) that is targeted to secretory pathway organelles (e.g., endoplasmic reticulum) by a hydrophobic carboxy terminus, and a 45 kDa isoform (TC45) that actively shuttles between the nucleus and cytoplasm (2). TCPTP substrates include receptor and non-receptor tyrosine kinases, such as EGFR, JAK1/3, and Src-family kinases, as well as STAT3 and other nuclear substrates (3-6). Research studies show that the corresponding PTPN2 gene is deleted in a subset of human T-cell acute lymphoblastic leukemias. The loss of TCPTP has been suggested to promote tumor progression through enhanced JAK/STAT signaling (7,8).

  1. Muppirala, M. et al. (2013) Biochim Biophys Acta 1833, 1125-32.
  2. Lorenzen, J.A. et al. (1995) J Cell Biol 131, 631-43.
  3. Simoncic, P.D. et al. (2002) Curr Biol 12, 446-53.
  4. Tiganis, T. et al. (1998) Mol Cell Biol 18, 1622-34.
  5. van Vliet, C. et al. (2005) Nat Immunol 6, 253-60.
  6. Fukushima, A. et al. (2010) Diabetes 59, 1906-14.
  7. Kleppe, M. et al. (2010) Nat Genet 42, 530-5.
  8. Kleppe, M. et al. (2011) Blood 117, 7090-8.

Application References

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Protocols

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For Research Use Only. Not For Use In Diagnostic Procedures.

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