Product Pathways - Lymphocyte Signaling
TCPTP (TC45) (D7T7D) Rabbit mAb #58935
|58935S||100 µl ( 10 western blots )||￥3,250.00 现货查询||购买询价|
|58935||carrier free & custom formulation / quantity||email request|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation,
Species predicted to react based on 100% sequence homology: Bovine, Dog, Pig,
Specificity / Sensitivity
TCPTP (TC45) (D7T7D) Rabbit mAb recognizes endogenous levels of total TCPTP (TC45) protein. This antibody does not recognize the 48 kDa (TC48) isoform.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro382 of human TCPTP (TC45) protein.
Western blot analysis of extracts from various cell lines using TCPTP (TC45) (D7T7D) Rabbit mAb.
T cell protein tyrosine phosphatase (TCPTP, PTPN2, PTN2) is a non-receptor protein tyrosine phosphatase (PTP) that regulates signal transduction pathways by catalyzing the dephosphorylation of tyrosine residues (1). Two described TCPTP splice variants include a 48 kDa isoform (TC48) that is targeted to secretory pathway organelles (e.g., endoplasmic reticulum) by a hydrophobic carboxy terminus, and a 45 kDa isoform (TC45) that actively shuttles between the nucleus and cytoplasm (2). TCPTP substrates include receptor and non-receptor tyrosine kinases, such as EGFR, JAK1/3, and Src-family kinases, as well as STAT3 and other nuclear substrates (3-6). Research studies show that the corresponding PTPN2 gene is deleted in a subset of human T-cell acute lymphoblastic leukemias. The loss of TCPTP has been suggested to promote tumor progression through enhanced JAK/STAT signaling (7,8).
- Muppirala, M. et al. (2013) Biochim Biophys Acta 1833, 1125-32.
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- Simoncic, P.D. et al. (2002) Curr Biol 12, 446-53.
- Tiganis, T. et al. (1998) Mol Cell Biol 18, 1622-34.
- van Vliet, C. et al. (2005) Nat Immunol 6, 253-60.
- Fukushima, A. et al. (2010) Diabetes 59, 1906-14.
- Kleppe, M. et al. (2010) Nat Genet 42, 530-5.
- Kleppe, M. et al. (2011) Blood 117, 7090-8.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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