Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

γ-Tubulin Antibody #5886

No. Size Price
5886S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
5886 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 50 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

γ-Tubulin Antibody recognizes endogenous levels of total γ-tubulin protein.

γ-Tubulin Antibody检测内源性γ-tubulin总蛋白。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human γ-tubulin protein. Antibodies are purified by protein A and peptide affinity chromatography.

通过人工合成人源γ-tubulin蛋白羧基端相应的多肽片段去免疫动物从而制备出多克隆抗体。通过蛋白A和多肽亲和层析纯化抗体。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using γ-Tubulin Antibody. Western blot analysis of extracts from various cell lines using γ-Tubulin Antibody.

使用γ-Tubulin Antibody,免疫印迹(Western Blot)分析不同细胞中γ-Tubulin。

Background

Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. As a critical part of the microtubule-organizing center (MTOC), the third member of the tubulin superfamily, γ-tubulin, is required for microtubule nucleation as well as centrosome duplication and spindle assembly (1,2, reviewed in 3). γ-tubulin forms complexes of two different sizes: γ-tubulin small complex (γ-TuSC) and the larger γ-tubulin ring complex (γ-TuRC). Each complex consists of a number of γ-tubulin complex proteins (GCPs) with γ-tubulin itself being considered GCP1. GCP2-6 all share sequence similarity in 5 different regions and it is thought that these areas could play a role in the proper folding of the proteins (4). γ-TuSC is composed of two γ-tubulin molecules as well as GCP2 and GCP3. γ-TuRC is made up of a ring of multiple copies of γ-TuSC in addition to GCP4, 5, and 6. Another protein, GCP-WD/NEDD1, which lacks sequence similarity with the other GCPs, associates with the γ-TuRC. GCP-WD/NEDD1 has been shown to regulate localization of the γ-TuSC to spindles and centrosomes (5-8). In mammals, phosphorylation of γ-tubulin at Ser131 by SADB controls the activity of the γ-TuRC. The hypothesis is that this phosphorylation stabilizes the protein in a conformation that stimulates centrosome amplification (9).

球形微管蛋白亚基包含微管建筑块,由α/β-tubulin异源二聚体形成所有真核细胞的tubulin亚单位。作为微管组织中心(microtubule organizing centers, MTOC)的一个关键成分,tubulin亚家族的第三个成员γ-tubulin蛋白对微管成核现象与中心体的复制和纺锤体的装配一样是需要的(1,2, 3)。γ-tubulin蛋白形成两个不同大小的复合物:γ-tubulin small complex (γ-TuSC)和比较大的γ-tubulin ring complex (γ-TuRC)。每一个复合物是由许多γ-tubulin复合物(GCPs)以及自身被考虑作为GCP1的γ-tubulin组成。GCP2-6在5个不同区域都含有序列相似性,并且被认为这些区域可能在该蛋白质的合适折叠中起到重要作用(4)。γ-TuSC是由 两个γ-tubulin分子也就是GCP2 和GCP3组成。除了GCP4, 5和6之外,γ-TuRC是多种γ-TuSC的组成。别的蛋白GCP-WD/NEDD1缺少与其它GCPs蛋白相同的序列,该蛋白与γ-TuRC有关联。GCP-WD/NEDD1蛋白已经证明去调节γ-TuSC定位到纺锤体和中心粒上(5-8)。在哺乳动物中,通过SADB使γ-tubulin蛋白在Ser131位点的磷酸化控制γ-TuRC的活性。一个假设就是该磷酸化能够稳定蛋白质构象,该过程能刺激中心粒的扩增(9)。

  1. Westermann, S. and Weber, K. (2003) Nat Rev Mol Cell Biol 4, 938-47.
  2. Loncarek, J. and Khodjakov, A. (2009) Mol Cells 27, 135-42.
  3. Wiese, C. and Zheng, Y. (2006) J Cell Sci 119, 4143-53.
  4. Murphy, S.M. et al. (2001) Mol Biol Cell 12, 3340-52.
  5. Raynaud-Messina, B. and Merdes, A. (2007) Curr Opin Cell Biol 19, 24-30.
  6. Schiebel, E. (2000) Curr Opin Cell Biol 12, 113-8.
  7. Lüders, J. et al. (2006) Nat Cell Biol 8, 137-47.
  8. Haren, L. et al. (2009) PLoS One 4, e5976.
  9. Alvarado-Kristensson, M. et al. (2009) Nat Cell Biol 11, 1081-92.

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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