Cell Signaling Technology

Product Pathways - Adhesion

Phospho-Desmoplakin (Ser165/166) Antibody #5885

No. Size Price
5885S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
5885 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 250 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Phospho-Desmoplakin (Ser165/166) Antibody recognizes endogenous levels of desmoplakin protein only when phosphorylated at Ser165/166.

Phospho-Desmoplakin (Ser165/166)抗体识别165/166位丝氨酸磷酸化后内源性desmoplakin蛋白。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser165/166 of human desmoplakin protein. Antibodies are purified by protein A and peptide affinity chromatography.

合成对应人desmoplakin蛋白165/166位丝氨酸及其附近氨基酸残基的多肽免疫动物获得多克隆抗体。抗体由蛋白A和肽亲和层析获得。

Western Blotting

Western Blotting

Western blot analysis of extracts from A-431 and Calu-3 cells, untreated or treated with calf intestinal phosphatase (CIP) and λ phosphatase, using Phospho-Desmoplakin (Ser165/166) Antibody.未处理或经过牛小肠碱性磷酸酶(CIP)和λ磷酸酶处理的A-431和Calu-3细胞提取物进行western blot分析,使用Phospho-Desmoplakin (Ser165/166)抗体进行实验。

Background

Desmosomes are a class of intracellular junction that tightly link adjacent cells in mechanically stressed tissues such as the epithelium and myocardium (1). They derive their characteristic strength from the protein Desmoplakin, which acts as a tether by binding the cytoplasmic component of the Desmosome at it’s N-terminus (2) while its C-terminus is anchored to the intermediate-filament cytoskeleton (3). This association mitigates the impact of mechanical forces on the desmosome by distributing them throughout the cytoskeleton and tissue (4). Desmoplakin is essential for normal desmosomal adhesion (5); defects can result in pathologies that include cardiomyopathy (6), keratoderma (7), or the skin blistering disease Epidermolysis bullosa (8).

桥粒是一种细胞连接,能够将机械压力组织中的上皮细胞和心肌细胞紧密连接(1)。Desmoplakin(桥粒斑蛋白)能够像身子一样发挥作用,其N端(2)与细胞内结构结合,C端锚定在细胞骨架中间丝(3)上从而使桥粒发挥功能。这种结合通过细胞骨架和组织传导能够缓冲机械力对桥粒的影响(4)。桥粒对于政策桥粒连接时必要的(5);桥粒缺陷会导致多种病变包括心肌病(6),皮肤角化病(7),或皮肤起庖的大庖性表皮松解症(8)。

Phospho-Desmoplakin (Ser165/166) Antibody is directed against a site that was identified at Cell Signaling Technology (CST) using PTMScan®, our LC-MS/MS platform for modification site discovery. Please visit PhosphoSitePlus®, a modification site knowledgebase developed and maintained by CST, at www.phosphosite.org for more information.

Cell Signaling Technology (CST) 使用PTMScan®识别的位点以设计Phospho-Desmoplakin (Ser165/166)抗体,本公司LC-MS/MS平台用于发现修饰位点。请访问PhosphoSitePlus®,一个由CST设计的修饰位点数据库,更多信息请访问www.phosphosite.org。

  1. Stokes, D.L. (2007) Curr Opin Cell Biol 19, 565-71.
  2. Kowalczyk, A.P. et al. (1997) J Cell Biol 139, 773-84.
  3. Meng, J.J. et al. (1997) J Biol Chem 272, 21495-503.
  4. Green, K.J. and Simpson, C.L. (2007) J Invest Dermatol 127, 2499-515.
  5. Vasioukhin, V. et al. (2001) Nat Cell Biol 3, 1076-85.
  6. Rampazzo, A. et al. (2002) Am J Hum Genet 71, 1200-6.
  7. Armstrong, D.K. et al. (1999) Hum Mol Genet 8, 143-8.
  8. Jonkman, M.F. et al. (2005) Am J Hum Genet 77, 653-60.

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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