Cell Signaling Technology

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Rabbit Anti-Mouse IgG (Light Chain Specific) (D3V2A) mAb (HRP Conjugate) #58802

anti mouse   anti-mouse   light chain   sc-2005   sc-2031   sc-2318   secondary  

No. Size Price
58802S 100 µl ( 10 western blots ) ¥3,986.00 现货查询 购买询价
58802 carrier free & custom formulation / quantityemail request
Applications Species-Reactivity Sensitivity MW (kDa) Isotype
W Endogenous Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Rabbit Anti-mouse IgG (Light Chain Specific) (D3V2A) mAb (HRP Conjugate) recognizes native mouse IgG. It also recognizes the denatured and reduced mouse IgG light chains (about 25 kDa) on a western blot.

Source / Purification

Monoclonal antibody is produced by immunizing animals with native total mouse IgG.

Description

This Cell Signaling Technology antibody is conjugated to the carbohydrate groups of horseradish peroxidase (HRP) via its amine groups.

Western Blotting

Western Blotting

Western blot analysis of decreasing concentrations of total mouse IgG reduced and denatured in 1x SDS loading buffer with DTT using Rabbit Anti-Mouse IgG (Light Chain Specific) (D3V2A) mAb (HRP Conjugate) (left) or Anti-Mouse IgG (HRP Conjugate) #7076 (right).

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells using β-Actin (8H10D10) Mouse mAb #3700 as the primary antibody. Rabbit Anti-mouse IgG (Light Chain Specific) (D3V2A) mAb (HRP Conjugate) (left) and Rabbit Anti-mouse IgG (HRP Conjugate) #7076 (right) secondary antibodies were used.

Background

Rabbit Anti-Mouse IgG (Light Chain Specific) (D3V2A) mAb (HRP Conjugate) only reacts with native IgG and denatured and reduced mouse IgG light chain. When performing immunopreciptiation (IP) followed by western blotting, the denatured mouse IgG heavy chains of the primary antibody used for IP run at approximately 50 kDa on the subsequent western blot and can often obscure bands of proteins that have similar molecular weights. Using Rabbit Anti-Mouse IgG (Light Chain Specific) (D3V2A) mAb (HRP Conjugate) as a secondary antibody will eliminate this problem.

Application References

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Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

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Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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