Product Pathways - Companion Products
Rabbit Anti-Mouse IgG (Light Chain Specific) (D3V2A) mAb (HRP Conjugate) #58802
|58802S||100 µl ( 10 western blots )||￥3,986.00 现货查询||购买询价|
|58802||carrier free & custom formulation / quantity||email request|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting,
Specificity / Sensitivity
Rabbit Anti-mouse IgG (Light Chain Specific) (D3V2A) mAb (HRP Conjugate) recognizes native mouse IgG. It also recognizes the denatured and reduced mouse IgG light chains (about 25 kDa) on a western blot.
Source / Purification
Monoclonal antibody is produced by immunizing animals with native total mouse IgG.
This Cell Signaling Technology antibody is conjugated to the carbohydrate groups of horseradish peroxidase (HRP) via its amine groups.
Western blot analysis of decreasing concentrations of total mouse IgG reduced and denatured in 1x SDS loading buffer with DTT using Rabbit Anti-Mouse IgG (Light Chain Specific) (D3V2A) mAb (HRP Conjugate) (left) or Anti-Mouse IgG (HRP Conjugate) #7076 (right).
Rabbit Anti-Mouse IgG (Light Chain Specific) (D3V2A) mAb (HRP Conjugate) only reacts with native IgG and denatured and reduced mouse IgG light chain. When performing immunopreciptiation (IP) followed by western blotting, the denatured mouse IgG heavy chains of the primary antibody used for IP run at approximately 50 kDa on the subsequent western blot and can often obscure bands of proteins that have similar molecular weights. Using Rabbit Anti-Mouse IgG (Light Chain Specific) (D3V2A) mAb (HRP Conjugate) as a secondary antibody will eliminate this problem.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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