Cell Signaling Technology

Product Pathways - Metabolism

FoxA1/HNF3α (D7P9B) Rabbit mAb #58613

forkhead box A1   Forkhead box protein A1   FOXA1   Hepatocyte nuclear factor 3-alpha   hepatocyte nuclear factor 3. alpha   HNF-3-alpha   HNF-3A   HNF3A   MGC33105   TCF-3A   TCF3A   Transcription factor 3A  

No. Size Price
58613S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
58613 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 50 Rabbit IgG
ChIP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, ChIP=Chromatin IP,

Specificity / Sensitivity

FoxA1/HNF3α (D7P9B) Rabbit mAb recognizes endogenous levels of total FoxA1/HNF3α protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu148 of human FoxA1/HNF3α protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using FoxA1/HNF3α (D7P9B) Rabbit mAb.

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 LNCaP cells and either 10 µl of FoxA1/HNF3α (D7P9B) Rabbit mAb or 2 µl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human KLK3 Promoter Primers #32784, human Slug exon 3 primers, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

Background

Forkhead box protein A1 (FoxA1, HNF3α) is a transcription factor required for the development of endoderm-derived organs, such as liver, lung, and prostate (1). FoxA1 functions as a pioneer factor that is recruited primarily to the distant enhancers to change chromatin structure for transcription in a cell type-specific manner (2,3). The FoxA1 transcription factor is implicated in various diseases, playing a role in hormone-dependent disorders such as breast and prostate cancers (4). The treatment of relapsing-remitting multiple sclerosis patients with IFN-β results in FoxA1-induced stimulation of a novel population of FoxA1(+) regulatory T-cells, suggesting a possible immunosuppressive role for FoxA1 (5).

  1. Friedman, J.R. and Kaestner, K.H. (2006) Cell Mol Life Sci 63, 2317-28.
  2. Lupien, M. et al. (2008) Cell 132, 958-70.
  3. Wang, A. et al. (2015) Cell Stem Cell 16, 386-99.
  4. Augello, M.A. et al. (2011) EMBO J 30, 3885-94.
  5. Liu, Y. et al. (2014) Nat Med 20, 272-82.

Application References

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Protocols

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

SimpleChIP is a registered trademark of Cell Signaling Technology, Inc.

Tween is a registered trademark of ICI Americas, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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