Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

Bmi1 (D42B3) Rabbit mAb #5856

polycomb   PRC1  

No. Size Price
5856S 100 µl ( 10 western blots ) ¥3,250.00 现货查询 购买询价 防伪查询
5856 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 41, 43 Rabbit IgG
IP 1:100
IF-IC 1:100
ChIP 1:50
ChIP-seq 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry), ChIP=Chromatin IP, ChIP-seq=Chromatin IP-seq,

Specificity / Sensitivity

Bmi1 (D42B3) Rabbit mAb recognizes endogenous levels of total Bmi1 protein.

Bmi1 (D42B3) Rabbit mAb兔单抗能够检测内源性Bmi1总蛋白水平。

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human Bmi1 protein.




Confocal immunofluorescent analysis of C2C12 cells using Bmi1 (D42B3) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red).

使用Bmi1 (D42B3) Rabbit mAb 兔单抗(绿色)标记,共聚焦免疫荧光分析C2C12细胞。DY-554 phalloidin标记微丝蛋白(红色)。

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 NCCIT cells and either 10 µl of Bmi1 (D42B3) Rabbit mAb or 2 µl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human HoxA1 intron 1 primers, SimpleChIP® Human HoxA2 Promoter Primers #5517, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin (equivalent to one).

使用SimpleChIP®Enzymatic Chromatin IP Kit (Magnetic Beads) #9003,用4 x 106 NCCIT细胞的交联染色质以及10 µl Bmi1 (D42B3) Rabbit mAb或2 µl Normal Rabbit IgG #2729进行染色质免疫沉淀实验。使用SimpleChIP®Human HoxA1 Intron 1 Primers #7707、SimpleChIP® Human HoxA2 Promoter Primers #5517和SimpleChIP® Human α Satellite Repeat Primers #4486,浓缩的DNA通过real-time PCR定量。在每个样品中免疫沉淀DNA的数量被当做一个相对于总input chromatin的数量的信号(相当于一个)。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Bmi1 (D42B3) Rabbit mAb.

使用Bmi1 (D42B3) Rabbit mAb兔单抗,免疫印迹(Western blot)分析不同细胞中Bmi1蛋白水平。

Chromatin IP-seq

Chromatin IP-seq

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 NCCIT cells and either 10 µl of Bmi1 (D42B3) Rabbit mAb or 10 μl of RING1B (D22F2) XP® Rabbit mAb #5694, using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. DNA Libraries were prepared from 5 ng enriched ChIP DNA using NEBNext® Ultra™ II DNA Library Prep Kit for Illumina®, and sequenced on the Illumina NextSeq. Bmi1 and RING1B are known to associate with each other on chromatin. The figure shows binding of both Bmi1 and RING1B across HOXD gene cluster, which are known target genes of Bmi1 and RING1B (see additional figure containing ChIP-qPCR data). For additional ChIP-seq tracks, please download the product data sheet.


The polycomb group (PcG) of proteins contributes to the maintenance of cell identity, stem cell self-renewal, cell cycle regulation, and oncogenesis by maintaining the silenced state of genes that promote cell lineage specification, cell death, and cell-cycle arrest (1-4). PcG proteins exist in two complexes that cooperate to maintain long-term gene silencing through epigenetic chromatin modifications. The first complex, EED-EZH2, is recruited to genes by DNA-binding transcription factors and methylates histone H3 on Lys27. This histone methyl-transferase activity requires the Ezh2, Eed, and Suz12 subunits of the complex (5). Histone H3 methylation at Lys27 facilitates the recruitment of the second complex, PRC1, which ubiquitinylates histone H2A on Lys119 (6). Bmi1 is a component of the PRC1 complex, which together with Ring1 strongly enhances the E3 ubiquitin ligase activity of the Ring2 catalytic subunit (7). Bmi1 plays an important role in the regulation of cell proliferation and senescence through repression of the p16 INK4A and p19 ARF genes and is required for maintenance of adult hematopoietic and neural stem cells (3,4,8-10).

polycomb group (PcG)蛋白有助于细胞身份的维持、干细胞自我更新、细胞周期调节和肿瘤形成,这些功能通过维持一些基因的沉默,而这些基因是促进细胞系特异性、细胞死亡和细胞周期停滞(1-4)。PcG蛋白存在两个复合物,它们配合通过表观遗传染色质修饰去维持长期的基因沉默。通过DNA结合转录因子和组蛋白H3在Lys27位点的甲基化,第一个复合物EED-EZH2被招募到基因上。这个组蛋白甲基转移酶活性需要该复合物的Ezh2、Eed和Suz12亚基(5)。组蛋白H3在Lys27位点的甲基化促进第二个复合物PRC1的招募,其能使组蛋白H2A在Lys119位点泛素化(6)。Bmi1蛋白是PRC1复合物的一个组成成分,该蛋白可与Ring1一起强有力的提高了Ring2催化亚基的E3泛素连接酶活性(7)。Bmi1蛋白在调节细胞增殖和衰老中起到重要作用,这主要通过p16 INK4A和p19 ARF基因的抑制,并且Bmi1蛋白对于成人造血和神经干细胞的维持是需要的(3,4,8-10)。

  1. Boyer, L.A. et al. (2006) Nature 441, 349-53.
  2. Lee, T.I. et al. (2006) Cell 125, 301-13.
  3. Park, I.K. et al. (2003) Nature 423, 302-5.
  4. Molofsky, A.V. et al. (2003) Nature 425, 962-7.
  5. Cao, R. and Zhang, Y. (2004) Mol Cell 15, 57-67.
  6. Wang, H. et al. (2004) Nature 431, 873-8.
  7. Cao, R. et al. (2005) Mol Cell 20, 845-54.
  8. Molofsky, A.V. et al. (2005) Genes Dev 19, 1432-7.
  9. Jacobs, J.J. et al. (1999) Nature 397, 164-8.
  10. Jacobs, J.J. et al. (1999) Genes Dev 13, 2678-90.

Application References

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