Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

MYST2 (D4N3F) Rabbit mAb #58418

No. Size Price
58418S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
58418 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 80 Rabbit IgG
IP 1:100
IHC-P 1:1000
ChIP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), ChIP=Chromatin IP,

Specificity / Sensitivity

MYST2 (D4N3F) Rabbit mAb recognizes endogenous levels of total MSYT2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein surrounding Val222 of human MYST2 protein.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using MYST2 (D4N3F) Rabbit mAb.

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 HCT 116 cells and either 10 μl of MYST2 (D4N3F) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human HoxA1 Intron 1 Primers #7707, SimpleChIP® Human HoxA9 Promoter Primers #14909, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

IP

IP

Immunoprecipitation of MYST2 from MOLT4 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is MYST2 (D4N3F) Rabbit mAb. Western blot analysis was performed using MYST2 (D4N3F) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using MYST2 (D4N3F) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using MYST2 (D4N3F) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using MYST2 (D4N3F) Rabbit mAb.

Background

MYST2, also known as HBO1 and lysine acetyltransferase 7 (KAT7), is a member of the MYST (MOZ, YBF2, SAS2 and Tip60) family of histone acetyltransferases (HATs). MYST2 is the catalytic subunit of the HBO1 acetyltransferase complex, which consists of MYST2, MYST/ESA1-asociated factor 6 (MEAF6), inhibitor of growth protein 4 (ING4) or inhibitor of growth protein 5 (ING5), and one of two families of scaffold proteins (JADE-1/2/3 or BRPF1/2/3) (1,2). The substrate specificity of the HBO1 complex is determined by the associated scaffold protein. HBO1 complexes containing JADE scaffold proteins acetylate histone H4 on lysines 5, 8 and 12, while complexes containing BRPF scaffold proteins acetylate histone H3 on lysines 14 and 23 (2). In addition the scaffold protein appears to regulate the function of the HBO1 complex. Complexes containing JADE scaffold proteins bind to origin recognition complex 1 (ORC1) and regulate licensing of DNA replication, while HBO1 complexes containing BRPF scaffold proteins regulate transcription (2-5). MYST2 is required for regulation of cell proliferation (1), adipogenesis (6), embryonic development (7) and survival of fetal liver erythroblasts (8). In addition, MYST2 is over-expressed in several human cancers, including cancers of the testis, ovary, breast, stomach, esophagus, and bladder (9). The MYST2 gene is amplified and protein is over-expressed in breast cancers, and over-expression of MYST2 increases anchorage-independent growth of several breast cancer cell lines (10).

  1. Avvakumov, N. et al. (2012) Mol Cell Biol 32, 689-703.
  2. Lalonde, M.E. et al. (2013) Genes Dev 27, 2009-24.
  3. Iizuka, M. and Stillman, B. (1999) J Biol Chem 274, 23027-34.
  4. Iizuka, M. et al. (2006) Mol Cell Biol 26, 1098-108.
  5. Miotto, B. and Struhl, K. (2010) Mol Cell 37, 57-66.
  6. Johmura, Y. et al. (2008) J Biol Chem 283, 2265-74.
  7. Kueh, A.J. et al. (2011) Mol Cell Biol 31, 845-60.
  8. Mishima, Y. et al. (2011) Blood 118, 2443-53.
  9. Iizuka, M. et al. (2009) Gene 436, 108-14.
  10. Hu, X. et al. (2009) Mol Cancer Res 7, 511-22.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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