Cell Signaling Technology

Product Pathways - Companion Products

Dinitrophenol (D1D6) Rabbit mAb (HRP Conjugate) #58203

Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:10000 All Species Expected, Transfected Only Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Dinitrophenol (D1D6) Rabbit mAb (HRP Conjugate) recognizes dinitrophenol-conjugated molecules.

Source / Purification

Monoclonal antibody is produced by immunizing animals with KLH-conjugated dinitrophenol.


This Cell Signaling Technology antibody is conjugated to the carbohydrate groups of horseradish peroxidase (HRP) via its amine groups. The HRP conjugated antibody is expected to exhibit the same species cross-reactivity as the unconjugated Dinitrophenol (D1D6) Rabbit mAb #14681.

Western Blotting

Western Blotting

Western blot analysis of dinitrophenol-conjugated BSA (BSA/DNP, 0.001 ng) using Dinitrophenol (D1D6) Rabbit mAb (HRP Conjugate).


The synthetic compound dinitrophenol (DNP) is a small hapten molecule that can be conjugated to proteins for use in immunoassays (1). Antibodies that recognize dinitrophenol will display strong binding affinity to DNP conjugated molecules, including nucleic acids and proteins. These DNP-conjugated compounds can be used in in situ hybridization, western, northern, and Southern blotting (1,2). Dinitrophenol is a commonly used insecticide and has been used as a treatment for weight loss (3).

  1. Grzybowski, J. et al. (1993) Nucleic Acids Res 21, 1705-12.
  2. Lehtovaara, P. et al. (1993) PCR Methods Appl 3, 169-75.
  3. Goldgof, M. et al. (2014) J Biol Chem 289, 19341-50.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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