Cell Signaling Technology

Product Pathways - Screening Technologies

Phospho-Thr-Pro-Pro Motif [pTPP] (D61C3) Rabbit mAb #5757

Motif   tpp  

No. Size Price
5757S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
5757 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 All Species Expected, Endogenous Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Phospho-Thr-Pro-Pro Motif [pTPP] (D61C3) Rabbit mAb recognizes endogenous levels of proteins only when phosphorylated within the TPP motif. It does not cross react with phospho-serine or phospho-threonine in other contexts.

Source / Purification

Monoclonal antibody is produced by immunizing animals with synthetic peptide library containing the phospho-Thr-Pro-Pro motif.

Western Blotting

Western Blotting

Western blot analysis of extracts from HCT 116 cells, G0 cells that were serum starved and contact inhibited (36 hr) or mitotic cells treated with thymidine (2 mM, 18 hr) followed by Nocodazole #2190 (50 ng/ml, 20 hr), using Phospho-Thr-Pro-Pro Motif [pTPP] (D61C3) Rabbit mAb (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).

Background

The phospho-Thr-Pro-Pro motif is a subgroup of the phospho-Thr-Pro motif that is phosphorylated by proline-directed protein kinases, including MAP kinases and cyclin dependent kinases (CDKs). Proline-directed phosphorylation is one of the major regulatory phosphorylation events in cell proliferation, cell differentiation, and a number of other essential cellular processes (1-6). This motif was identified in both phospho-proteomic and motif X analyses as a significant phospho-thr motif (7,8).

Application References

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Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

Use of Cell Signaling Technology (CST) Motif Antibodies within certain methods (e.g., U.S. Patents No. 7,198,896 and 7,300,753) may require a license from CST. For information regarding academic licensing terms please have your technology transfer office contact CST Legal Department at CST_ip@cellsignal.com. For information regarding commercial licensing terms please contact CST Pharma Services Department at ptmscan@cellsignal.com.

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