Product Pathways - Screening Technologies
Phospho-Thr-Pro-Pro Motif [pTPP] (D61C3) Rabbit mAb #5757
|5757S||100 µl ( 10 western blots )||￥3,900.00 现货查询||购买询价|
|5757||carrier free & custom formulation / quantity||email request|
|W||1:1000||All Species Expected,||Endogenous||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting,
Specificity / Sensitivity
Phospho-Thr-Pro-Pro Motif [pTPP] (D61C3) Rabbit mAb recognizes endogenous levels of proteins only when phosphorylated within the TPP motif. It does not cross react with phospho-serine or phospho-threonine in other contexts.
Source / Purification
Monoclonal antibody is produced by immunizing animals with synthetic peptide library containing the phospho-Thr-Pro-Pro motif.
Western blot analysis of extracts from HCT 116 cells, G0 cells that were serum starved and contact inhibited (36 hr) or mitotic cells treated with thymidine (2 mM, 18 hr) followed by Nocodazole #2190 (50 ng/ml, 20 hr), using Phospho-Thr-Pro-Pro Motif [pTPP] (D61C3) Rabbit mAb (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).
The phospho-Thr-Pro-Pro motif is a subgroup of the phospho-Thr-Pro motif that is phosphorylated by proline-directed protein kinases, including MAP kinases and cyclin dependent kinases (CDKs). Proline-directed phosphorylation is one of the major regulatory phosphorylation events in cell proliferation, cell differentiation, and a number of other essential cellular processes (1-6). This motif was identified in both phospho-proteomic and motif X analyses as a significant phospho-thr motif (7,8).
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For Research Use Only. Not For Use In Diagnostic Procedures.
Use of Cell Signaling Technology (CST) Motif Antibodies within certain methods (e.g., U.S. Patents No. 7,198,896 and 7,300,753) may require a license from CST. For information regarding academic licensing terms please have your technology transfer office contact CST Legal Department at CST_ip@cellsignal.com. For information regarding commercial licensing terms please contact CST Pharma Services Department at firstname.lastname@example.org.
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