Cell Signaling Technology

Product Pathways - MAPK Signaling

Phospho-SAPK/JNK (Thr183/Tyr185) (G9) Mouse mAb (PE Conjugate) #5755

No. Size Price
5755S 100 µl ( 50 tests ) ¥3,986.00 现货查询 购买询价
5755 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
F 1:50 Human,Mouse,Rat,Hamster,S. cerevisiae, Endogenous Mouse IgG1

Species cross-reactivity is determined by western blot.

Applications Key: F=Flow Cytometry,

Specificity / Sensitivity

Phospho-SAPK/JNK (Thr183/Tyr185) (G9) Mouse mAb (PE Conjugate) detects endogenous levels of p46 and p54 SAPK/JNK dually phosphorylated at Thr183 and Tyr185. This antibody does not recognize endogenous levels of phosphorylated p44/42 MAPK or p38 MAPK.

Phospho-SAPK/JNK (Thr183/Tyr185) (G9) Mouse mAb (PE Conjugate)鼠单抗能检测内源性能检测内源性Thr183和Tyr185双磷酸化的p46 、p54 SAPK/JNK蛋白水平。该抗体不能识别磷酸化的p44/42、p38 MAPK。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr183/Tyr185 of human SAPK/JNK. The antibody is purified by affinity chromatography.

该单克隆抗体是采用合成的与人源SAPK/JNK蛋白苏Thr183/Tyr185位点周围残基相一致的磷酸化肽段免疫动物而获得。该抗体经亲和层析纯化。

Description

This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Phospho-SAPK/JNK (Thr183/Tyr185) (G9) Mouse mAb #9255.

该抗体偶联了藻红蛋白(PE),并经过了内部测试,可用于人类细胞样本的流式细胞分析检测。预期该抗体与未偶联的抗体Phospho-SAPK/JNK (Thr183/Tyr185) (G9) Mouse mAb #9255具有相同的物种交叉反应性。


  

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of THP-1 cells, untreated (blue) or anisomycin-treated (green), using Phospho-SAPK/JNK (Thr183/Tyr185) (G9) Mouse mAb (PE Conjugate).流式细胞方法检测未处理(蓝色)和茴香霉素处理(绿色)的THP-1细胞,蓝色为未处理组,绿色为处理组。使用抗体为Phospho-SAPK/JNK (Thr183/Tyr185) (G9) Mouse mAb (连接PE)。

Background

The stress-activated protein kinase/Jun-amino-terminal kinase SAPK/JNK is potently and preferentially activated by a variety of environmental stresses including UV and gamma radiation, ceramides, inflammatory cytokines, and in some instances, by growth factors and GPCR agonists (1-6). As with the other MAPKs, the core signaling unit is composed of a MAPKKK, typically MEKK1-MEKK4, or by one of the mixed lineage kinases (MLKs), which phosphorylate and activate MKK4/7. Upon activation, MKKs phosphorylate and activate the SAPK/JNK kinase (2). Stress signals are delivered to this cascade by small GTPases of the Rho family (Rac, Rho, cdc42) (3). Both Rac1 and cdc42 mediate the stimulation of MEKKs and MLKs (3). Alternatively, MKK4/7 can be activated in a GTPase-independent mechanism via stimulation of a germinal center kinase (GCK) family member (4). There are three SAPK/JNK genes each of which undergoes alternative splicing resulting in numerous isoforms (3). SAPK/JNK, when active as a dimer, can translocate to the nucleus and regulate transcription through its effects on c-Jun, ATF-2, and other transcription factors (3,5).

应激活化蛋白激酶/Jun氨基末端激酶SAPK/JNK能够有效、优先地被多种环境压力激活,这些环境压力包括紫外照射和gamma 辐射、神经酰胺、炎性因子,在某些情况下也包括生长因子和GPCR激动剂 (1-6)。正如其他的 MAPKs,核心信号传导单元包括一个MAPKKK,通常是MEKK1-MEKK4,或一种混合谱系酶 (MLKs),这些MAPKKK激酶能磷酸化并激活 MKK4/7。活化后,MKKs磷酸化并激活SAPK/JNK 激酶 (2)。应激反应信号经Rho 家族(Rac, Rho, cdc42)的小GTP酶被传递到这个级联信号反应中 (3)。Rac1 和 cdc42蛋白都能调节MEKKs、 MLKs的激活(3)。另外一种情况是,MKK4/7能够通过生发中心激酶 (GCK)家族成员的刺激,在一个非GTP酶依赖机制中被激活 (4)。有三种不同的SAPK/JNK基因,而每一种都会经过选择性剪切产生多种异构体(3)。活化的二聚体SAPK/JNK,能转位到细胞核中,通过它对 c-Jun, ATF-2和其他转录因子的效应而调节转录过程(3,5)。

  1. Davis, R.J. (1999) Biochem Soc Symp 64, 1-12.
  2. Ichijo, H. (1999) Oncogene 18, 6087-93.
  3. Kyriakis, J.M. and Avruch, J. (2001) Physiol Rev 81, 807-69.
  4. Kyriakis, J.M. (1999) J Biol Chem 274, 5259-62.
  5. Leppä, S. and Bohmann, D. (1999) Oncogene 18, 6158-62.
  6. Whitmarsh, A.J. and Davis, R.J. (1998) Trends Biochem Sci 23, 481-5.

Application References

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Protocols


For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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