Cell Signaling Technology

Product Pathways - Tyrosine Kinase / Adaptors

EGF Receptor (E746-A750del Specific) (D6B6) XP® Rabbit mAb (Biotinylated) #5747

No. Size Price
5747S 100 µl ( 10 western blots ) ¥4,264.00 现货查询 购买询价
5747 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 175 Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

EGF Receptor (E746-A750del Specific) (6B6) XP® Rabbit mAb (Biotinylated) detects endogenous levels of EGFR E746-A750del mutant protein.

EGF Receptor (E746-A750缺失特异) (6B6) XP® 兔单抗(生物素标记)能够检测内源性水平的EGFR E746-A750缺失突变蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to E746-A750del mutant sequence of human EGFR.

该单克隆抗体是通过用合成肽免疫动物而制备的,该合成肽是人EGFR的E746-A750缺失突变序列。

Description

This Cell Signaling Technology antibody is conjugated to biotin under optimal conditions. The biotinylated antibody is expected to exhibit the same species cross-reactivity as the unconjugated EGF Receptor (E746-A750del Specific) (6B6) XP® Rabbit mAb #2085.

该Cell Signaling Technology抗体在最佳条件下与生物素结合。该生物素化抗体预期与未结合的EGF Receptor (E746-A750del Specific) (6B6) XP® 兔单抗 #2085表现出同一物种的交叉反应。

Western Blotting

Western Blotting

Western blot analysis of extracts from HCC827 and H3255 cells using EGF Receptor (E746-A750del Specific) (6B6) XP® Rabbit mAb (Biotinylated).Western blot方法检测HCC827细胞和H3255细胞提取物,使用的抗体为EGF Receptor (E746-A750del Specific) (6B6) XP® Rabbit mAb (Biotinylated).

Background

The epidermal growth factor (EGF) receptor is a 170 kDa transmembrane tyrosine kinase that belongs to the HER/ErbB protein family. Research studies have shown that somatic mutations in the tyrosine kinase domain of EGF receptor (EGFR) are present in a subset of lung adenocarinomas that respond to EGFR inhibitors, such as gefinitib and erlotinib (1-3). Two types of mutations account for approximately 90% of mutated cases: a specific point mutation, L858R, that occurs in exon 21 and short in-frame deletions in exon 19 (4,5). The most frequent exon 19 deletion is E746-A750, accounting for 90% of lesions at this site, although some rare variants occur.

表皮生长因子(EGF)受体是一种170kDa的跨膜酪氨酸激酶,属于HER/ErbB蛋白家族。调查研究表明,EGF受体(EGFR)酪氨酸激酶结构域的体细胞突变存在于非小细胞肺癌子集,即对EGFR抑制剂吉非替尼、厄洛替尼有反应的子集(1-3)。有两种类型的突变占突变的情况约90%:一个特定的点突变,在外显子21的L858R突变和外显子19中的框内缺失(4,5)。虽然出现一些罕见的变异,但最常见的19号外显子缺失是E746-A750,占该位点病变的90%。

  1. Lynch, T.J. et al. (2004) N Engl J Med 350, 2129-39.
  2. Pao, W. et al. (2004) Proc Natl Acad Sci USA 101, 13306-11.
  3. Haber, D.A. et al. (2005) Cold Spring Harb Symp Quant Biol 70, 419-26.
  4. Kosaka, T. et al. (2004) Cancer Res 64, 8919-23.
  5. Riely, G.J. et al. (2006) Clin Cancer Res 12, 7232-41.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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