Cell Signaling Technology

Product Pathways - Tyrosine Kinase / Adaptors

EGF Receptor (E746-A750del Specific) (D6B6) XP® Rabbit mAb (Biotinylated) #5747

No. Size Price
5747S 100 µl ( 10 western blots ) ¥4,264.00 现货查询 购买询价
5747 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 175 Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

EGF Receptor (E746-A750del Specific) (6B6) XP® Rabbit mAb (Biotinylated) detects endogenous levels of EGFR E746-A750del mutant protein.

EGF Receptor (E746-A750缺失特异) (6B6) XP® 兔单抗(生物素标记)能够检测内源性水平的EGFR E746-A750缺失突变蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to E746-A750del mutant sequence of human EGFR.



This Cell Signaling Technology antibody is conjugated to biotin under optimal conditions. The biotinylated antibody is expected to exhibit the same species cross-reactivity as the unconjugated EGF Receptor (E746-A750del Specific) (6B6) XP® Rabbit mAb #2085.

该Cell Signaling Technology抗体在最佳条件下与生物素结合。该生物素化抗体预期与未结合的EGF Receptor (E746-A750del Specific) (6B6) XP® 兔单抗 #2085表现出同一物种的交叉反应。

Western Blotting

Western Blotting

Western blot analysis of extracts from HCC827 and H3255 cells using EGF Receptor (E746-A750del Specific) (6B6) XP® Rabbit mAb (Biotinylated).Western blot方法检测HCC827细胞和H3255细胞提取物,使用的抗体为EGF Receptor (E746-A750del Specific) (6B6) XP® Rabbit mAb (Biotinylated).


The epidermal growth factor (EGF) receptor is a 170 kDa transmembrane tyrosine kinase that belongs to the HER/ErbB protein family. Research studies have shown that somatic mutations in the tyrosine kinase domain of EGF receptor (EGFR) are present in a subset of lung adenocarinomas that respond to EGFR inhibitors, such as gefinitib and erlotinib (1-3). Two types of mutations account for approximately 90% of mutated cases: a specific point mutation, L858R, that occurs in exon 21 and short in-frame deletions in exon 19 (4,5). The most frequent exon 19 deletion is E746-A750, accounting for 90% of lesions at this site, although some rare variants occur.


  1. Lynch, T.J. et al. (2004) N Engl J Med 350, 2129-39.
  2. Pao, W. et al. (2004) Proc Natl Acad Sci USA 101, 13306-11.
  3. Haber, D.A. et al. (2005) Cold Spring Harb Symp Quant Biol 70, 419-26.
  4. Kosaka, T. et al. (2004) Cancer Res 64, 8919-23.
  5. Riely, G.J. et al. (2006) Clin Cancer Res 12, 7232-41.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0


我要参与评论 :