Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

ELP3 (D5H12) Rabbit mAb #5728

No. Size Price
5728S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
5728 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 62 Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,


Species predicted to react based on 100% sequence homology: Hamster, Bovine, Dog, Pig, Horse,

Specificity / Sensitivity

ELP3 (D5H12) Rabbit mAb recognizes endogenous levels of total ELP3 protein.

ELP3 (D5H12) Rabbit mAb检测内源性ELP3总蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly431 of human ELP3 protein.


Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using ELP3 (D5H12) Rabbit mAb.

使用ELP3 (D5H12) Rabbit mAb兔单抗,免疫印迹(Western blot)分析不同细胞中ELP3 (D5H12)蛋白水平。


Elongator is a highly conserved transcription elongation factor complex that was first identified in yeast as part of the hyperphosphorylated RNA polymerase II (RNAPII) holoenzyme (1). The Elongator complex consists of 6 subunits, ELP1-6, and has been shown to have acetyltransferase activity (2). The acetylation targets of Elongator include histone H3, which is linked to the transcription elongation function of the complex, and α-tubulin, which is associated with regulation of migration and maturation of projection neurons (3-6).

ELP3 is the catalytic acetyltransferase subunit of the Elongator complex (2,3). ELP3 contains an iron-sulfur cluster that can bind S-adenosylmethionine, which is necessary for the structural integrity of the Elongator and has been shown to play an important role in mediating global zygotic DNA demethylation of the paternal genome post-fertilization (7-9).

Elongator是一个高度保守的转录延伸因子复合物,它首先在酵母中被鉴定,并且作为高度磷酸化的RNA polymerase II (RNAPII)全酶的部分(1)。Elongator复合物是由6个亚单位ELP1-6组成,并且研究显示有乙酰化转移酶活性 (2)。Elongator乙酰化靶点包含 histone H3和α-tubulin,而histone H3是联系着该复合物的转录延伸功能,以及α-tubulin是与迁移的调节和投射神经元的成熟有关(3-6)。

ELP3是Elongator复合物的催化乙酰转移酶亚单位(2,3)。ELP3 包含一个铁硫簇,它能结合S-adenosylmethionine,这对于Elongator复合物完整性是必要的,并且研究显示在介导父系基因组受精作用之后的整个受精卵DNA去甲基化(7-9)。

  1. Otero, G. et al. (1999) Mol Cell 3, 109-18.
  2. Creppe, C. and Buschbeck, M. (2011) J Biomed Biotechnol 2011, 924898.
  3. Wittschieben, B.O. et al. (1999) Mol Cell 4, 123-8.
  4. Hawkes, N.A. et al. (2002) J Biol Chem 277, 3047-52.
  5. Kim, J.H. et al. (2002) Proc Natl Acad Sci USA 99, 1241-6.
  6. Creppe, C. et al. (2009) Cell 136, 551-64.
  7. Paraskevopoulou, C. et al. (2006) Mol Microbiol 59, 795-806.
  8. Greenwood, C. et al. (2009) J Biol Chem 284, 141-9.
  9. Okada, Y. et al. (2010) Nature 463, 554-8.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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