Cell Signaling Technology

Product Pathways - Growth Factors/Cytokines

Mouse His6Interleukin-27 (mHis6IL-27) #5725

No. Size Price
5725SC 10 µg ( With Carrier ) ¥2,580.00 现货查询 购买询价
5725SF 10 µg ( Carrier Free ) ¥2,580.00 现货查询 购买询价
5725 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype

Species cross-reactivity is determined by western blot.

Applications Key:

Source / Purification

Recombinant mouse His6IL-27 (mHis6IL-27) is a heterodimer that is composed of EBI3 Tyr19-Pro228 (Accession# NP_056581) linked to p28 Phe29-Ser234 (Accession# NP_663611) via the linker GGGSGGGSGGGSGGGS. mHis6IL-27 was expressed in human 293 cells at Cell Signaling Technology.

CST公司生产的重组小鼠蛋白His6IL-27 (mHis6IL-27)是异源二聚体,由EBI3 Tyr19-Pro228 (Accession# NP_056581)和p28 Phe29-Ser234 (Accession# NP_663611)通过 GGGSGGGSGGGSGGGS连接而成。此蛋白在人的293细胞中表达。

Molecular Characterization

Recombinant N-terminally His6-tagged mIL-27 has a calculated MW of 50,551. DTT-reduced and non-reduced protein migrate as 55 kDa polypeptides. The expected amino terminus of recombinant mHis6IL-27 was verified by amino acid sequencing.

重组的mIL-27蛋白在N-末端有His6标签,蛋白分子量据推算为50,551Da。DTT-还原的蛋白和未还原的蛋白作为55kDa的多肽而迁移。重组蛋白的N-末端序列通过测序来证实。

Purity

>97% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant mHis6IL-27. All lots are greater than 97% pure.

6 μg 还原 (+) 和 非还原的(-) 重组mHis6IL-27通过SDS-PAGE检测,纯度大于97%,所有批次的纯度均高于97%。

Bioactivity

The bioactivity of mHis6IL-27 was determined in a virus protection assay. The ED50 of each lot is between 1-6 ng/ml.

mHis6IL-27 的生物活性是通过病毒保护实验来确定的。每个批次的ED50在1-6 ng/ml之间。

Bioactivity

Bioactivity

The bioactivity of recombinant mHis6IL-27 was determined in a virus protection assay. Hep G2 cells were pre-treated with increasing concentrations of mHis6IL-27 for 24 hr. Cells were then innoculated with encephalomyocarditis virus (EMCV) and incubated for an additional 24 hr. Surviving cells were fixed and stained with crystal violet and the OD595 was determined.重组蛋白mHis6IL-27的生物活性是通过病毒保护实验确定的。Hep G2细胞用浓度递增的mHis6IL-27前处理24小时然后与脑心肌炎病毒(EMCV)共孵育另外的24小时。存活的细胞固定然后用结晶紫并测定OD595 数值。

Bioactivity

Bioactivity

The bioactivity of recombinant mHis6IL-27 was determined by induction of MxA mRNA. Hep G2 cells were pre-treated with increasing concentrations of mHis6IL-27 for 5 hr. Induction of MxA gene expression was determined by qPCR.重组蛋白mHis6IL-27的生物活性是通过诱导MxA mRNA的实验确定的。Hep G2细胞用浓度递增的mHis6IL-27前处理5小时。MxA基因的表达是通过qPCR来确定。

Coomassie Gel

Coomassie Gel

The purity of recombinant mHis6IL-27 was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant mHis6IL-27 and staining overnight with Coomassie Blue.重组mHis6IL-27蛋白的纯度通过SDS-PAGE 来确定。6 µg 经还原(+)和未经过还原(-) 的重组mHis6IL-27蛋白跑SDS胶并用考马斯亮蓝染色过夜。

Western Blotting

Western Blotting

Western blot analysis of extracts from Hep G2 cells, untreated or treated with mHis6IL-27 for 15 min, using Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb #9145 (upper) or Stat3 Antibody #9132 (lower).Western免疫印迹。用Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb #9145 (上图)和 Stat3 Antibody #9132 (下图) 研究未经处理的和经mHis6IL-27处理15分钟的Hep G2 细胞的细胞提取液。

Endotoxin

Less than 0.01 ng endotoxin/1μg mHis6IL-27.

每微克mHis6IL-27内毒素含量小于0.01 ng。

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg mHis6IL-27. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.

有载体:每微克mHis6IL-27蛋白溶解在包含 20 μg BSA的PBS(pH 7.2)溶液中,并通过 0.22 μm 滤膜冻干。

无载体:每微克mHis6IL-27蛋白溶解在PBS(pH 7.2)溶液中,并通过 0.22 μm 滤膜冻干。

Background

IL-27 is a heterodimer that consists of p28 and EBI3 (1). Antigen presenting cells including monocytes, macrophages, and dendritic cells are the primary sources of IL-27 (1). IL-27 is a pleiotropic cytokine with both pro- and anti-inflammatory activities. IL-27 suppresses TH17 responses and promotes type I regulatory cell differentiation (2-4). In contrast, IL-27 has been shown to play a pro-inflammatory role in a mouse model of T cell-induced colitis (5). IL-27 may also have anti-viral activities (6). IL-27 exerts its effects through a heterodimeric receptor consisting of WSX-1 and gp130 (1,7). IL-27-induced signaling results in Stat1 and Stat3 phosphorylation (1,7).

IL-27是由p28和EBI3组成的异源二聚体(1)。抗原表达细胞包括单核细胞,巨噬细胞和树突状细胞,这些细胞都是IL-27最主要的来源(1)。IL-27是一个多向性的细胞因子,具有促炎症和抗炎症的双重活性。IL-27抑制TH17的应答并促进I型调节细胞分化的调控(2-4)。相反,IL-27在T细胞诱导的肠炎小鼠模型中显示了重要的促炎作用(5)。IL-27可能也具有抗病毒的活性(6)。IL-27发挥其作用主要是通过由WSX-1和gp130组成的异源二聚体受体(1,7)。IL-27诱导的信号通路包括Stat1和Stat3的磷酸化(1,7)。

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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