Cell Signaling Technology

Product Pathways - Tyrosine Kinase / Adaptors

Phospho-Axl (Tyr702) (D12B2) Rabbit mAb #5724

No. Size Price
5724S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
5724 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 138 Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Homology

Species predicted to react based on 100% sequence homology: Mouse, Rat,

Specificity / Sensitivity

Phospho-Axl (Tyr702) (D12B2) Rabbit mAb detects endogenous levels of Axl only when phosphorylated at Tyr702. This antibody may also cross-react with other overexpressed, related tyrosine-phosphorylated tyrosine kinases, such as EGFR. 磷酸化的Axl (Tyr702)的兔源单克隆抗体仅在Tyr702被磷酸化后才能检测到Axl的存在。本抗体也能与其它过表达的相关磷酸化酪氨酸激酶(如EGFR)交叉反应。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr702 of human Axl protein. 单克隆抗体通过免疫动物得到,该合成磷酸化多肽是根据人的Axl蛋白Tyr702附近的氨基酸序列合成的。

Western Blotting

Western Blotting

Western blot analysis of extracts from NCI-H1299 cells, untreated or Gas6-treated (100 ng/ml for 10 minutes), using Phospho-Axl (Tyr702) (D12B2) Rabbit mAb (upper) or Axl (C44G1) Rabbit mAb #4566 (lower). 用抗磷酸化的Axl (Tyr702)的兔源单克隆抗体(上)或抗Axl (C44G1) 的兔源单克隆抗体(下)对经过或未经Gas6 (100 ng/ml , 10分钟)处理的NCI-H1299细胞的提取物进行免疫印迹检测。

Background

Axl, Sky and Mer are three members of a receptor tyrosine kinase (RTK) family that share a conserved intracellular tyrosine kinase domain and an extracellular domain similar to those seen in cell adhesion molecules. These RTKs bind the vitamin K-dependent protein growth-arrest-specific 6 (Gas6), which is structurally related to the protein S anticoagulation factor (1). Upon binding to its receptor, Gas6 activates phosphatidylinositol 3-kinase (PI3K) and its downstream targets Akt and S6K, as well as NF-κB (2,3). A large body of evidence supports a role for Gas6/Axl signaling in cell growth and survival in normal and cancer cells (4).

Axl、 Sky和 Mer是 受体酪氨酸激酶 (RTK) 家族的三个成员,它们有共同的胞内酪氨酸激酶区域和与胞外粘附分子相似的胞外区。这些受体酪氨酸激酶与维生素K依赖性的生长停滞特异性6蛋白(Gas6)结合,Gas6蛋白与S抗凝血因子蛋白结构上相关(1)。与受体结合后,Gas6能够激活PI3K激酶和它的下游靶分子Akt、 S6K和 NF-κB (2,3)。大量证据表明Gas6/Axl信号通路与正常细胞和癌细胞的生长和存活相关(4)。

Phosphorylation of Axl Receptor on Tyr702 was identified at Cell Signaling Technology (CST) using PhosphoScan®, CST's LC-MS/MS platform for phosphorylation site discovery (5). Phosphorylation of Axl on Tyr702 was also reported in select carcinoma cell lines (6).

Axl的Tyr1702位点磷酸化是Cell Signaling Technology (CST)公司利用LC-MS/MS平台PhosphoScan®技术在寻找磷酸化位点过程中发现的(5)。Axl的Tyr702位点磷酸化在某些肿瘤细胞系中存在(6)。

  1. Crosier, K.E. and Crosier, P.S. (1997) Pathology 29, 131-5.
  2. Demarchi, F. et al. (2001) J Biol Chem 276, 31738-44.
  3. Lee, W.P. et al. (2002) Oncogene 21, 329-36.
  4. Bellosta, P. et al. (1997) Oncogene 15, 2387-97.
  5. Rikova, K. et al. (2007) Cell 131, 1190-203.
  6. Guo, A. et al. (2008) Proc Natl Acad Sci USA 105, 692-7.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0

该产品暂无评论!

我要参与评论 :

如要参与评论请先登录网站

还没有网站账户?去注册一下吧

Products

 

Applications