Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

SMC3 (D47B5) Rabbit mAb #5696

cohesin   cohesion  

No. Size Price
5696S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
5696 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:2000 Human,Mouse,Rat,Monkey, Endogenous 140 Rabbit IgG
IP 1:200
IF-IC 1:200

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry),

Homology

Species predicted to react based on 100% sequence homology: Chicken, Xenopus, Zebrafish, Bovine,

Specificity / Sensitivity

SMC3 (D47B5) Rabbit mAb recognizes endogenous levels of total SMC protein. In immunofluorescent analysis, this antibody shows nuclear staining as expected; however, it also shows weak cytoplasmic staining that is presumed to be background. SMC3 (D47B5) Rabbit mAb能够识别内源性SMC总蛋白的水平。在免疫荧光分析中,该抗体显示了预期的核染;然而,它也显示了预测为背景的弱的胞质染色。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg132 of human SMC3 protein. 该单克隆抗体是由合成的针对人SMC3蛋白的132位赖氨酸的肽段免疫动物生产的。

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells using SMC3 (D47B5) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). 激光共聚焦荧光法检测 HeLa 细胞,使用的抗体为SMC3 (D47B5) Rabbit mAb (绿色)。肌动蛋白纤维丝使用DY-554 鬼笔环肽标记(红色)。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using SMC3 (D47B5) Rabbit mAb. Western blot方法检测各种细胞提取物,使用的抗体为SMC3 (D47B5) Rabbit mAb。

Background

The cohesin complex consists of a heterodimer between SMC1 (SMC1A or B) and SMC3, bound by additional RAD21 and STAG proteins (STAG1, 2, or 3) (1,2). These proteins form a ring-like structure that mediates the cohesion of two sister chromatids after DNA replication in S phase (1,2). RAD21 and STAG2 are phosphorylated by Polo-like kinase (PLK) during prophase, which leads to the dissociation of cohesin complexes from the chromosome arms; however, cohesin remains bound to centromeres until anaphase (3,4). RAD21 is cleaved by separin/ESPL1 in anaphase, which leads to dissociation of the remaining cohesin from centromeres, enabling sister chromatids to segregate during mitosis (5). RAD21 is also cleaved by caspase-3 and caspase-7 during apoptosis, resulting in a 64 kDa carboxy-terminal cleavage product that translocates to the cytoplasm and may help to trigger apoptosis (6,7). In addition to mediating cohesion of sister chromatids, the cohesin complex plays important roles in gene regulation and DNA repair, as SMC1 and SMC3 are both phosphorylated by ATM and ATR kinases upon DNA damage (1,2). 黏连蛋白复合物是由介于SMC1 (SMC1A or B)和SMC3之间的异源二聚体组成,邻接RAD21和STAG蛋白(STAG1,2或3) (1,2)。这些蛋白形成一个环状结构来介导S期DNA复制后姐妹染色单体的结合(1,2)。RAD21和STAGE2可在分裂前期被Polo样激酶(PLK)磷酸化,导致黏连蛋白复合体从染色体臂分离;然而,黏连蛋白仍然结合中中心体直至分裂后期(3,4)。RAD21可以在分裂后期被分离蛋白/ESPL1裂解,导致剩下的黏连蛋白从中心体分离,促进有丝分裂中姐妹染色单体的分离(5)。RAD21也能够在凋亡中被凋亡蛋白酶-3和凋亡蛋白酶-7裂解,导致一个64kDa的羧基末端裂解产物向胞质转移并有助于触发凋亡(6,7)。除了介导姐妹染色单体的结合,黏连蛋白复合物在基因调控和DNA修复中发挥重要作用,因为在DNA损伤处SMC1和SMC3都被ATM和ATR激酶磷酸化(1,2)。

  1. Peters, J.M. et al. (2008) Genes Dev 22, 3089-114.
  2. Barbero, J.L. (2009) Cell Mol Life Sci 66, 2025-35.
  3. Hoque, M.T. and Ishikawa, F. (2001) J Biol Chem 276, 5059-67.
  4. Hauf, S. et al. (2005) PLoS Biol 3, e69.
  5. Hauf, S. et al. (2001) Science 293, 1320-3.
  6. Pati, D. et al. (2002) Mol Cell Biol 22, 8267-77.
  7. Chen, F. et al. (2002) J Biol Chem 277, 16775-81.

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