Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

DBC1 Antibody #5693

breast cancer   DBC-1   DBC.1   deleted in breast cancer gene 1   p30 DBC  

No. Size Price
5693S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
5693 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 130 Rabbit
IP 1:50
IF-IC 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry),

Homology

Species predicted to react based on 100% sequence homology: Horse,

Specificity / Sensitivity

DBC1 Antibody detects endogenous levels of total DBC1 protein.

DBC1 Antibody能够检测内源性DBC1总蛋白水平。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human DBC1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

通过合成的与人源DBC1蛋白氨基端相应的多肽片段去免疫动物从而制备出此多克隆抗体。通过蛋白A和多肽亲和层析纯化获得。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using DBC1 Antibody.

使用DBC1 Antibody,免疫印迹(Western blot)分析不同细胞中DBC1的蛋白水平。

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells using DBC1 Antibody (green). Actin filaments were labeled with DY-554 phalloidin (red).

使用DBC1 Antibody (绿色)标记,共聚焦免疫荧光分析HeLa细胞。DY-554 phalloidin标记微丝蛋白(红色)。

Background

Deleted in breast cancer gene 1 protein (DBC1) was originally identified by its localization to a region of chromosome 8p21 that is homozygously deleted in breast cancer (1). DBC1 is a large, nuclear protein with multiple functions in cell survival. It binds directly to the estrogen receptor α (ERα) hormone-binding domain in a ligand-independent manner and may be a key determinant of ligand-independent ERα expression and survival in human breast cancer cells (2). DBC1 can promote p53-mediated apoptosis by binding to and inhibiting the deacetylase activity of SirT1, resulting in increased p53 acetylation levels and activity (3). DBC1 may be an important regulator of heterochromatin formation as it binds SUV39H1 and inhibits its histone methyltransferase activity (4). Caspase-dependent processing activates the pro-apoptotic activity of DBC1 during Tumor Necrosis Factor-α (TNF-α)-mediated cell death signaling (5). This processing of DBC1 in response to TNF-α is an early event in the onset of apoptosis and results in relocalization of DBC1 to the cytoplasm. Overexpression of the processed, cytoplasmic form of DBC1 results in mitochondrial clustering and matrix condensation and sensitizes cells to TNF-α-mediated apoptosis.

Deleted in breast cancer gene 1 protein (DBC1)最初通过它定位到染色体8p21区域被鉴定,它在乳腺癌中纯合子的缺失(1)。在细胞存活中,DBC1蛋白是一个具有多种功能的分子量大的细胞核蛋白。它以非配体依赖性方式直接结合到雌激素受体 (strogen receptor α,ERα)激素结合区域,并且可能是非配体ERα 表达和存在人源乳腺癌细胞系中的一个关键因素(2)。DBC1蛋白可能通过结合到和抑制SirT1蛋白的去乙酰酶活性从而促进p53介导的凋亡,这导致p53乙酰化水平和活性的增加(3)。DBC1蛋白可能是一个重要异染色质形成的调节因子,因为它结合SUV39H1以及抑制它的组蛋白甲基转移酶活性(4)。在Tumor Necrosis Factor-α (TNF-α)介导的细胞死亡信号期间,Caspase依赖的进程可以激活DBC1的促凋亡活性(5)。在TNF-α刺激下,DBC1蛋白的这个过程发生在凋亡的初期,并且导致DBC1蛋白重新定位到细胞质。DBC1蛋白的加工的细胞质形式的过量表达导致线粒体的群集和基质浓缩,并且使的细胞对于TNF-α介导凋亡敏感。

  1. Hamaguchi, M. et al. (2002) Proc Natl Acad Sci USA 99, 13647-52.
  2. Trauernicht, A.M. et al. (2007) Mol Endocrinol 21, 1526-36.
  3. Zhao, W. et al. (2008) Nature 451, 587-90.
  4. Li, Z. et al. (2009) J Biol Chem 284, 10361-6.
  5. Sundararajan, R. et al. (2005) Oncogene 24, 4908-20.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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