Cell Signaling Technology

Product Pathways - Lineage Markers

Oct-4A (C30A3C1) Rabbit mAb (ChIP Formulated) #5677

No. Size Price
5677S 100 µl ( 10 immunoprecipitations ) ¥3,100.00 现货查询 购买询价
5677 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
ChIP 1:50 Human,Mouse, Endogenous Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: ChIP=Chromatin IP,

Specificity / Sensitivity

Oct-4A (C30A3C1) Rabbit mAb (ChIP Formulated) detects endogenous levels of total Oct-4A protein.

Oct-4A (C30A3C1)Rabbit mAb兔单抗(ChIP 级)识别内源性的总Oct-4A蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human Oct-4A protein.

单克隆抗体是通过合成的肽段免疫动物产生。这种合成的肽段与邻近人源Oct-4A蛋白氨基末端的氨基酸序列一致。

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 NCCIT cells and either 10 μl of Oct-4A (C30A3C1) Rabbit mAb (ChIP Formulated) or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human Oct-4 Promoter Primers #4641, SimpleChIP® Human Sox2 Promoter Primers #4649, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

使用SimpleChIP® Enzymatic Chromatin IP Kit (磁珠偶联) #9003将4 x 106 NCCIT细胞和10μl Oct-4A (C30A3C1)Rabbit mAb兔单抗(ChIP级别)或2μl Normal Rabbit IgG#2779进行染色质免疫共沉淀。富集到的DNA随后使用SimpleChIP® Human Oct-4启动子引物#4641,SimpleChIP® 人Sox2启动子引物#4649和SimpleChIP® 人α卫星重复序列引物#4486进行荧光实时PCR定量。各样品沉淀得到的DNA量显示比对input染色质总量进行相对定量,Input染色质量设定值为1。

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 mES cells and either 10 μl of Oct-4A (C30A3C1) Rabbit mAb (ChIP Formulated) or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Mouse Oct-4 Promoter Primers #4653, SimpleChIP® Mouse XIST Intron 1 Primers #4659, and SimpleChIP® Mouse RPL30 Intron 2 Primers #7015. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

使用SimpleChIP® Enzymatic Chromatin IP Kit (磁珠偶联) #9003将4 x 106 小鼠胚胎干细胞和10μl Oct-4A (C30A3C1)Rabbit mAb兔单抗(ChIP级别)或2μl Normal Rabbit IgG#2779进行染色质免疫共沉淀。富集到的DNA随后使用SimpleChIP®小鼠 Oct-4启动子引物#4653,SimpleChIP® Mouse XIST内含子1引物#4659和SimpleChIP®鼠RPL30内含子2引物#7015进行荧光实时PCR定量。各样品沉淀得到的DNA量显示比对input染色质总量进行相对定量,Input染色质量设定值为1。

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 NTERA-2 cells and either 10 μl of Nanog (D73G4) XP® Rabbit mAb (ChIP Formulated) #5232, Oct-4A (C30A3C1) Rabbit mAb (ChIP Formulated) #5677, Sox2 (D6D9) XP® Rabbit mAb (ChIP Formulated), or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human Nanog promoter primers, SimpleChIP® Human Oct-4 Promoter Primers #4641, SimpleChIP® Human Sox2 Promoter Primers #4649, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

使用SimpleChIP® Enzymatic Chromatin IP Kit (磁珠偶联) #9003将4 x 106 NTERA-2细胞和Nanog(D73G4)XP® 兔单抗(ChIP级别)#5232,Oct-4A (C30A3C1)兔单抗(ChIP级别),Sox2 (D6D9) XP® 兔单抗(ChIP级别)或2μl Normal Rabbit IgG#2779进行染色质免疫共沉淀。富集到的DNA随后使用人Nanog启动子引物,SimpleChIP® 人Oct-4启动子引物#4641,SimpleChIP® 人Sox2启动子引物#4649和SimpleChIP® 人α卫星重复序列引物#4486进行荧光实时PCR定量,Input染色质量设定值为1。

Background

Oct-4 (POU5F1) is a transcription factor highly expressed in undifferentiated embryonic stem cells and embryonic germ cells (1). A network of key factors that includes Oct-4, Nanog, and Sox2 is necessary for the maintenance of pluripotent potential, and downregulation of Oct-4 has been shown to trigger cell differentiation (2,3). Oct-4 is a useful germ cell tumor marker (4). Oct-4 exists as two splice variants, Oct-4A and Oct-4B (5). Recent studies have suggested that it is the Oct-4A isoform that has the ability to confer and sustain pluripotency and that Oct-4B may exist in some somatic, non-pluripotent cells (6,7).

Oct-4 (POU5F1)是一类高表达于未分化胚胎干细胞和原始胚胎生殖细胞中的转录因子(1),一系列关键因子包括Oct-4, Nanog和 Sox2对保持多潜能性是必需的,Oct-4的下调显示能起始细胞分化(2,3)。Oct-4是一种非常有用的生殖细胞肿瘤标志物[4]。Oct-4存在两种剪接异构体Oct-4A and Oct-4B(5)。近来研究暗示Oct-4A异构体能给予并保持干细胞多潜能,而Oct-4B可能存在于某些体细胞和非多潜能性细胞中(6,7)。

  1. Looijenga, L.H. et al. (2003) Cancer Res 63, 2244-50.
  2. Pesce, M. and Schöler, H.R. (2001) Stem Cells 19, 271-278.
  3. Pan, G. and Thomson, J.A. (2007) Cell Res 17, 42-9.
  4. Cheng, L. et al. (2007) J Pathol 211, 1-9.
  5. Takeda, J. et al. (1992) Nucleic Acids Res 20, 4613-20.
  6. Cauffman, G. et al. (2006) Stem Cells 24, 2685-91.
  7. Lee, J. et al. (2006) J Biol Chem 281, 33554-65.

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For Research Use Only. Not For Use In Diagnostic Procedures.

U.S. Patent No. 5,675,063.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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