Cell Signaling Technology

Product Pathways - Neuroscience

β3-Tubulin (D65A4) XP® Rabbit mAb #5666

beta-3-Tubulin   beta-III-tubulin   TU-20   TUJ-1   βtubulin  

No. Size Price
5666S 100 µl ( 10 western blots ) ¥3,580.00 现货查询 购买询价
5666T 20 µl ( 2 western blots ) ¥1,400.00 现货查询 购买询价
5666 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 55 Rabbit IgG
IP 1:50
IHC-P 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin),

Specificity / Sensitivity

β3-Tubulin (D65A4) XP® Rabbit mAb detects endogenous levels of total β3-tubulin protein. This antibody does not cross-react with tubulin isoforms expressed in non-neuronal cells. This clone is similar to TUJ1.

β3-Tubulin (D65A4) XP® Rabbit mAb 兔单抗识别内源性的总β3-微管蛋白。此抗体不与非神经元内表达的微管蛋白发生交叉反应。此克隆类似TUJ1。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the carboxy terminus of human β3-tubulin protein.


Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines and tissues using β3-Tubulin (D65A4) XP® Rabbit mAb (upper) and α/β-Tubulin Antibody #2148 (lower).

Western blot分析多种细胞系和组织提取物,使用的抗体是β3-Tubulin(D65A4)XP® Rabbit mAb 兔单抗(上图)及α/β-Tubulin Antibody 兔多抗 #2148(下图)。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded rat cerebellum using β3-Tubulin (D65A4) XP® Rabbit mAb.

免疫组化分析石蜡包埋的大鼠小脑,使用的抗体是β3-Tubulin(D65A4)XP® Rabbit mAb 兔单抗。


The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. γ-tubulin is required to nucleate polymerization of tubulin subunits to form microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from competitive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins (1).


  1. Westermann, S. and Weber, K. (2003) Nat Rev Mol Cell Biol 4, 938-47.
  2. Jiang, Y.Q. and Oblinger, M.M. (1992) J Cell Sci 103 ( Pt 3), 643-51.
  3. Panda, D. et al. (1994) Proc Natl Acad Sci U S A 91, 11358-62.
  4. Tischfield, M.A. et al. (2010) Cell 140, 74-87.
  5. Katsetos, C.D. et al. (2003) J Child Neurol 18, 851-66; discussion 867.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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