Cell Signaling Technology

Product Pathways - Neuroscience

Musashi-1 (D46A8) Rabbit mAb #5663

sc-98845  

No. Size Price
5663S 100 µl ( 10 western blots ) ¥3,580.00 现货查询 购买询价
5663T 20 µl ( 2 western blots ) ¥1,400.00 现货查询 购买询价
5663 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 35 Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Musashi-1 (D46A8) XP® Rabbit mAb detects endogenous levels of total Musashi-1 protein.

Musashi-1 (D46A8) XP® Rabbit mAb 兔单抗识别内源性的总Musashi-1蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Met190 of human Musashi-1 protein.

该单克隆抗体由合成的人类Musashi-1蛋白Met190位点附近肽段免疫动物而制成。

IF-F

IF-F

Confocal immunofluorescent analysis of P4 rat brain using Musashi-1 (D46A8) XP® Rabbit mAb (green) and Nestin (Rat-401) Mouse mAb #4760 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

共聚焦免疫荧光分析P4大鼠大脑,使用的抗体是Musashi-1 (D46A8) XP® Rabbit mAb 兔单抗(绿色)和Nestin (Rat-401) Mouse mAb 鼠单抗 #4760(红色)。蓝色伪彩=DRAQ5® #4084 (荧光DNA染料)。

Western Blotting

Western Blotting

Western blot analysis of extracts from neonatal mouse brain and fetal rat brain using Musashi-1 (D46A8) XP® Rabbit mAb.

Western blot分析新生小鼠和胎大鼠大脑提取物,使用的抗体是Musashi-1 (D46A8) XP® Rabbit mAb 兔单抗。

Western Blotting

Western Blotting

Western blot analysis of extracts from SH-SY5Y, NCCIT, and PC-3 cells using Musashi-1 (D46A8) XP® Rabbit mAb (upper) and α/β-Tubulin Antibody #2148 (lower).

Western blot分析SH-SY5Y、NCCIT和PC-3细胞提取物,使用的抗体是Musashi-1 (D46A8) XP® Rabbit mAb 兔单抗(上图)及α/β-Tubulin Antibody 兔多抗#2148(下图)。

Background

Musashi-1 and Musashi-2 are RNA-binding proteins which play a role in asymmetric cell division of ectodermal precursor cells by regulating the translation of target mRNA. Both family members augment Notch signaling and repress the translation of m-Numb, a protein that positively modulates differentiation of neural stem cells into neurons. Thus, Musashi contributes to the maintenance of neural stem cells (1). While Musashi-1 is frequently used as a marker for proliferating neural precursor cells, it is also expressed in epithelial stem cells including intestinal and mammary gland stem cells (2-4).

Musashi-1和Musashi-2是RNA结合蛋白,通过调节靶mRNA的翻译在外胚层前体细胞的不对称细胞分裂中发挥作用。这两个成员都能增加Notch信号和抑制m-Numb的翻译;m-Numb是一种正向调节神经干细胞分化成神经元的蛋白质。因此,Musashi对神经干细胞的保存有贡献(1)。Musashi-1经常被用来作为神经前体细胞增殖的标记,它也在上皮干细胞,包括肠,乳腺干细胞中表达(2-4)。

  1. Okano, H. et al. (2005) Exp. Cell Res. 306, 349-56.
  2. Sakakibara, S. et al. (1996) Dev. Biol. 176, 230-42.
  3. Potten, C.S. et al. (2003) Differentiation 71, 28-41.
  4. Clarke, R.B. et al. (2005) Dev. Biol. 277, 443-56.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

DRAQ5 is a registered trademark of Biostatus Limited.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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