Cell Signaling Technology

Product Pathways - Development

Phospho-β-Catenin (Ser552) (D8E11) Rabbit mAb #5651

catenin   CTNNB1  

No. Size Price
5651S 100 µl ( 10 western blots ) ¥4,050.00 现货查询 购买询价 防伪查询
5651T 20 µl ( 2 western blots ) ¥1,500.00 现货查询 购买询价 防伪查询
5651 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse, Endogenous 92 Rabbit IgG
IP 1:200

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,


Species predicted to react based on 100% sequence homology: Rat, Xenopus, Zebrafish,

Specificity / Sensitivity

Phospho-β-Catenin (Ser552) (D8E11) Rabbit mAb detects endogenous levels of β-catenin protein only when phosphorylated at Ser552.

磷酸化-β-Catenin (丝氨酸552) (D8E11)兔单克抗只识别Ser552磷酸化的内源β-Catenin蛋白。

Source / Purification

Monoclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser552 of human β-catenin protein.


Western Blotting

Western Blotting

Western blot analysis of extracts from SK-N-MC and NTERA-2 cells, untreated or λ phosphatase-treated, usingPhospho-β-Catenin (Ser552) (D8E11) Rabbit mAb (upper) or β-Catenin (6B3) Rabbit mAb #9582 (lower).

未经处理或经λ 碱性磷酸酶处理后的SK-N-MC和NTERA-2细胞,使用usingPhospho-β-Catenin (Ser552) (D8E11) 兔单抗(上) 或 β-Catenin (6B3) 兔单抗 #9582 (下)进行western blot实验.


β-Catenin is a key downstream effector in the Wnt signaling pathway (1). It is implicated in two major biological processes in vertebrates: early embryonic development (2) and tumorigenesis (3). CK1 phosphorylates β-catenin at Ser45. This phosphorylation event primes β-catenin for subsequent phosphorylation by GSK-3β (4-6). GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41 (7). Mutations at these sites result in the stabilization of β-catenin protein levels and have been found in many tumor cell lines (8).

β-catenin是Wnt信号通路下游的重要效应分子(1)。在脊椎动物体内它涉及到两个重要的生物过程:早期胚胎发育(2)和肿瘤发生(3)。CK1可以磷酸化β-catenin 45位丝氨酸。该磷酸化将导致β-catenin能够随后被GSK-3磷酸化(4-6)。GSK-3β通过磷酸化β-catenin Ser33, Ser37, 和 Thr41,并促使其进一步被降解(7)。这些位点发生突变会提高β-catenin蛋白稳定性,并且已在多种癌细胞株中发现这些突变(8)。

Both Akt and PKA were shown to phosphorylate β-catenin at Ser552. Phosphorylation at Ser552 induces β-catenin accumulation in the nucleus and increases its transcriptional activity (9-11).

Akt 和 PKA均显示能磷酸化β-catenin的 552位丝氨酸。552位丝氨酸的磷酸化诱导β-catenin的核内累积并增加其转导活性[9-11].

  1. Cadigan, K.M. and Nusse, R. (1997) Genes Dev 11, 3286-305.
  2. Wodarz, A. and Nusse, R. (1998) Annu Rev Cell Dev Biol 14, 59-88.
  3. Polakis, P. (1999) Curr Opin Genet Dev 9, 15-21.
  4. Amit, S. et al. (2002) Genes Dev 16, 1066-76.
  5. Liu, C. et al. (2002) Cell 108, 837-47.
  6. Yanagawa, S. et al. (2002) EMBO J 21, 1733-42.
  7. Yost, C. et al. (1996) Genes Dev 10, 1443-54.
  8. Morin, P.J. et al. (1997) Science 275, 1787-90.
  9. Taurin, S. et al. (2006) J Biol Chem 281, 9971-9976.
  10. Fang, D. et al. (2007) J Biol Chem 282, 11221-11229.
  11. He, X.C. et al. (2007) Nat Genet 39, 189-198.

Application References

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