Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

BCL6 (D65C10) Rabbit mAb #5650

B-cell lymphoma 6   sc-858  

No. Size Price
5650S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
5650 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse, Endogenous 87-98 Rabbit IgG
IP 1:200
ChIP 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, ChIP=Chromatin IP,

Specificity / Sensitivity

BCL6 (D65C10) Rabbit mAb recognizes endogenous levels of total BCL6 protein.

BCL6 (D65C10) Rabbit mAb 兔单抗能够检测内源水平BCL6蛋白总体水平。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant protein specific to human BCL6 protein.

此单克隆抗体是通过合成人源对应的 BCL6 融合蛋白来免疫动物而获得。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using BCL6 (D65C10) Rabbit mAb.Western免疫印迹。用BCL6 (D65C10) Rabbit mAb研究各种细胞系的细胞提取液。

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 Raji cells and either 5 µl of BCL6 (D65C10) Rabbit mAb, or 2 µl of Normal Rabbit IgG #2729, using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human BCL2 Promoter Primers #12924, SimpleChIP® Human HSP90B1 Promoter Primers #12899, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.


Chromosomal translocations result in misregulation of the proto-oncogene BCL6 in patients with B cell-derived non-Hodgkins lymphoma (1). The BCL6 gene is selectively expressed in mature B cells and encodes a nuclear phosphoprotein that belongs to the BTB/POZ zinc finger family of transcription factors (2,3). BCL6 protein can bind to target DNA sequences of Stat6 and, analogous to Stat6, modulate the expression of interleukin-4-induced genes (4). Furthermore, BCL6 restrains p53-dependent senescence, making BCL6-active tumors functionally p53-negative (5). The mitogen-activated protein kinases, Erk1 and Erk2, but not JNK, phosphorylate BCL6 at multiple sites. Phosphorylation of BCL6 at Ser333 and Ser343 results in degradation of BCL6 by the ubiquitin/proteasome pathway in B cells (6,7). In addition, BCL6 is acetylated and its transcriptional repressor function is inhibited by the transcriptional co-activator p300 (8).

在B细胞起源的非霍奇金淋巴瘤病人中,染色体易位导致了对原癌基因Bcl-6 的调节失去准确性(1)。 The Bcl-6在成熟的B细胞中选择性表达并编码了属于BTB/POZ锌指蛋白家族转录调控因子的核磷酸化蛋白(2-3)。Bcl-6 蛋白能结合到Stat6蛋白和同源蛋白的目标DNA序列,调节白细胞介素-4诱导基因的表达(4)。另外,Bcl-6抑制了p53依赖的衰老,使得Bcl-6激活肿瘤功能上p53成为负因素(5)。分裂原活化蛋白激酶Erk1和Erk2, 而不是JNK, 在多位点磷酸化 Bcl-6。在B细胞中,磷酸化位于Bcl-6的Ser333 和 Ser343位点导致了Bcl-6被泛素/蛋白酶体通路降解(6-7)。另外, Bcl-6 被乙酰化后其转录抑制的功能也被转录共激活因子p300所抑制(8)。

  1. Ye, B.H. et al. (1993) Science 262, 747-50.
  2. Onizuka, T. et al. (1995) Blood 86, 28-37.
  3. Zollman, S. et al. (1994) Proc Natl Acad Sci U S A 91, 10717-21.
  4. Harris, M.B. et al. (1999) Mol Cell Biol 19, 7264-75.
  5. Shvarts, A. et al. (2002) Genes Dev 16, 681-6.
  6. Moriyama, M. et al. (1997) Oncogene 14, 2465-74.
  7. Niu, H. et al. (1998) Genes Dev 12, 1953-61.
  8. Bereshchenko, O.R. et al. (2002) Nat Genet 32, 606-13.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0


我要参与评论 :