Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

MTA1 (D40D1) XP® Rabbit mAb #5647

nurd complex  

No. Size Price
5647S 100 µl ( 10 western blots ) ¥3,750.00 现货查询 购买询价
5647T 20 µl ( 2 western blots ) ¥1,400.00 现货查询 购买询价
5647 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 78-82 Rabbit IgG
IHC-P 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin),

Specificity / Sensitivity

MTA1 (D40D1) XP® Rabbit mAb recognizes endogenous levels of total MTA1 protein.

MTA1 (D40D1) XP® Rabbit mAb兔单抗能够检测内源性MTA1总蛋白水平。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human MTA1 protein.


Western Blotting

Western Blotting

Western blot analysis of extracts from MCF7 and A-204 cells using MTA1 (D40D1) XP® Rabbit mAb.

使用MTA1 (D40D1) XP® Rabbit mAb兔单抗,免疫印迹(Western blot)分析MCF7和A-204细胞中MTA1 (D40D1)的蛋白水平。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using MTA1 (D40D1) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).

使用MTA1 (D40D1) XP® Rabbit mAb,control peptide (左图)或antigen-specific peptide (右图),免疫组化分析人源肺癌组织石蜡切片。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded mouse 4T1A mouse syngeneic tumor using MTA1 (D40D1) XP® Rabbit mAb.

使用MTA1 (D40D1) XP® Rabbit mAb,免疫组化分析小鼠4T1A mouse syngeneic tumor组织石蜡切片。


MTA1 (metastasis associated gene 1) was identified in a differential screening of a cDNA library of metastatic and nonmetastatic adenocarcinoma cell lines (1), and was subsequently found to be an integral member of the nucleosome remodeling and deacetylation (NuRD) complex (2,3). MTA1 expression is upregulated under hypoxic conditions and found to enhance angiogenesis through stabilization of HIF-1α (4,5). MTA1 is overexpressed in a wide range of human cancers, and its expression is associated with malignancy and tumor progression (6). MTA1 is an essential downstream effector of c-Myc transformation (7). Recently, MTA1 was demonstrated to play a role in DNA damage response (8,9).

MTA1 (metastasis associated gene 1)在转移性和非转移性腺癌细胞系的 cDNA文库的差异筛选中被鉴定(1),并且随后被发现是核小体重塑和去乙酰化(NuRD)复合物的完整成员(2,3)。MTA1蛋白表达在缺氧条件下被上调,并且发现通过HIF-1α的稳定性能提高血管生成 (4,5)。MTA1在大范围的人源癌症中过表达,以及它的表达是与恶性肿瘤和肿瘤进展有关(6)。MTA1是一个本质的c-Myc转化的下游效应分子(7)。最近,MTA1被证明在DNA损伤反应中起着一定作用(8,9)。

  1. Toh, Y. et al. (1994) J Biol Chem 269, 22958-63.
  2. Xue, Y. et al. (1998) Mol Cell 2, 851-61.
  3. Zhang, Y. et al. (1998) Cell 95, 279-89.
  4. Yoo, Y.G. et al. (2006) EMBO J 25, 1231-41.
  5. Moon, H.E. et al. (2006) Oncol Rep 16, 929-35.
  6. Toh, Y. and Nicolson, G.L. (2009) Clin Exp Metastasis 26, 215-27.
  7. Zhang, X.Y. et al. (2005) Proc Natl Acad Sci U S A 102, 13968-73.
  8. Li, D.Q. et al. (2009) J Biol Chem 284, 34545-52.
  9. Li, D.Q. et al. (2010) J Biol Chem 285, 10044-52.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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