Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

MTA1 (D17G10) Rabbit mAb #5646

nurd complex  

No. Size Price
5646S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
5646 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 78-82 Rabbit IgG
IP 1:200
ChIP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, ChIP=Chromatin IP,

Specificity / Sensitivity

MTA1 (D17G10) Rabbit mAb detects endogenous levels of total MTA1 protein.

MTA1 (D17G10) Rabbit mAb兔单抗能够检测内源性MTA1的总蛋白水平。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val632 of human MTA1 protein.

通过合成的与人源MTA1蛋白Val632位点周围相应多肽片段去免疫动物从而制备出此单克隆抗体。

Western Blotting

Western Blotting

Western blot analysis of extracts from MCF7 and A-204 cells using MTA1 (D17G10) Rabbit mAb.

使用MTA1 (D17G10) Rabbit mAb,免疫印迹(Western blot)分析MCF7和A-204细胞中MTA1 (D17G10)的蛋白水平。

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 K562 cells and either 10 µl of MTA1 (D17G10) XP® Rabbit mAb or 2 µl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human RPL30 Exon 3 Primers #7014, human BCR exon 1 primers, and SimpleChIP® Human Nanog Promoter Primers #95064. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

Background

MTA1 (metastasis associated gene 1) was identified in a differential screening of a cDNA library of metastatic and nonmetastatic adenocarcinoma cell lines (1), and was subsequently found to be an integral member of the nucleosome remodeling and deacetylation (NuRD) complex (2,3). MTA1 expression is upregulated under hypoxic conditions and found to enhance angiogenesis through stabilization of HIF-1α (4,5). MTA1 is overexpressed in a wide range of human cancers, and its expression is associated with malignancy and tumor progression (6). MTA1 is an essential downstream effector of c-Myc transformation (7). Recently, MTA1 was demonstrated to play a role in DNA damage response (8,9).

MTA1 (metastasis associated gene 1)在转移性和非转移性腺癌细胞系的 cDNA文库的差异筛选中被鉴定(1),并且随后被发现是核小体重塑和去乙酰化(NuRD)复合物的完整成员(2,3)。MTA1蛋白表达在缺氧条件下被上调,并且发现通过HIF-1α的稳定性能提高血管生成 (4,5)。MTA1在大范围的人源癌症中过表达,以及它的表达是与恶性肿瘤和肿瘤进展有关(6)。MTA1是一个本质的c-Myc转化的下游效应分子(7)。最近,MTA1被证明在DNA损伤反应中起着一定作用(8,9)。

  1. Toh, Y. et al. (1994) J Biol Chem 269, 22958-63.
  2. Xue, Y. et al. (1998) Mol Cell 2, 851-61.
  3. Zhang, Y. et al. (1998) Cell 95, 279-89.
  4. Yoo, Y.G. et al. (2006) EMBO J 25, 1231-41.
  5. Moon, H.E. et al. (2006) Oncol Rep 16, 929-35.
  6. Toh, Y. and Nicolson, G.L. (2009) Clin Exp Metastasis 26, 215-27.
  7. Zhang, X.Y. et al. (2005) Proc Natl Acad Sci U S A 102, 13968-73.
  8. Li, D.Q. et al. (2009) J Biol Chem 284, 34545-52.
  9. Li, D.Q. et al. (2010) J Biol Chem 285, 10044-52.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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