Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

Phospho-TACC3 (Ser558) Antibody #5645


No. Size Price
5645S 100 µl ( 10 western blots ) ¥4,050.00 现货查询 购买询价 防伪查询
5645 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 140 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Phospho-TACC3 (Ser558) Antibody detects endogenous levels of TACC3 protein only when phosphorylated at Ser558. Phospho-TACC3 (Ser558) Antibody能够检测内源性558位丝氨酸磷酸化的TACC3蛋白水平。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser558 of human TACC3 protein. Antibodies are purified by Protein A and peptide affinity chromatography. 该多克隆抗体是由合成的针对人TACC3蛋白558位丝氨酸的肽段免疫动物生产的。该抗体通过A蛋白和多肽亲和层析纯化。

Western Blotting

Western Blotting

Western blot analysis of extracts from HT-29 and U-2 OS cells, untreated or synchronized in mitosis by thymidine block (2 mM, 16 hours) followed by nocodozole treatment (100 ng/ml, 24 hours), using Phospho-TACC3 (Ser558) Antibody. Western blot 方法检测HT-29和U-2 OS 细胞提取物,未处理或者紧接着诺考达唑处理(100 ng/ml, 24 hours)胸腺嘧啶阻遏(2 mM, 16 hours) 引起有丝分裂同步化,使用的抗体为Phospho-TACC3 (Ser558) Antibody。


Transforming acid coiled-coil (TACC) proteins are a family of proteins characterized by a common coiled-coil motif of approximately 200 amino acids at the carboxy-terminal end (1). Three family members have been identified in humans: TACC1, TACC2, and TACC3. These proteins are thought to be involved in centrosomal microtubule assembly and have been mapped to chromosomal regions that are disrupted in some cancers (reviewed in 2). TACC3 has been shown to be upregulated in many cancer cell lines (3). When phosphorylated at Ser558 by Aurora A, mammalian TACC3 is localized to mitotic spindles and increases microtubule stability (4,5). For this reason, it has been suggested that monitoring the localization of phosphorylated TACC3 would be an effective way to determine the efficacy of Aurora A inhibitors that show promise as anti-cancer drugs (6,7). In addition, studies have shown that TACC3 could be useful as a prognostic marker for non-small cell lung cancer (8). 转化酸性卷曲螺旋蛋白(TACC)是一个蛋白家族,其特征是羧基末端共有的约200个氨基酸的卷曲螺旋模体(1)。人类中已经鉴定出三个家族成员:TACC1,TACC2和TACC3。这些蛋白被认为参与中心体微管装配且其染色体区域定位在一些癌症中是被破坏的(2中已论述)。TACC3被认为在多种癌细胞系中表达上调(3)。当其558位丝氨酸被极光激酶A磷酸化时,哺乳动物TACC3向有丝分裂纺锤体定位且增加微管的稳定性(4,5)。鉴于此,监控磷酸化TACC3的定位将是确定极光激酶A抑制剂效力的有效方式,而极光激酶A抑制剂是很有前景的抗癌药物(6,7)。此外,研究发现TACC3是一个有效的非小细胞肺癌的预后标志物(8)。

  1. Gergely, F. et al. (2000) Proc Natl Acad Sci USA 97, 14352-7.
  2. Peset, I. and Vernos, I. (2008) Trends Cell Biol 18, 379-88.
  3. Still, I.H. et al. (1999) Genomics 58, 165-70.
  4. Kinoshita, K. et al. (2005) J Cell Biol 170, 1047-55.
  5. Schneider, L. et al. (2007) J Biol Chem 282, 29273-83.
  6. LeRoy, P.J. et al. (2007) Cancer Res 67, 5362-70.
  7. Tyler, R.K. et al. (2007) Cell Cycle 6, 2846-54.
  8. Jung, C.K. et al. (2006) Pathol Int 56, 503-9.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0


我要参与评论 :