Cell Signaling Technology

Product Pathways - Tyrosine Kinase / Adaptors

Phospho-TNK1 (Tyr277) (D46E7) Rabbit mAb #5638

No. Size Price
5638S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
5638 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 72 TNK1, 58 TNK1-C17orf61 Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,


Species predicted to react based on 100% sequence homology: Mouse, Rat,

Specificity / Sensitivity

Phospho-TNK1 (Tyr277) (D46E7) Rabbit mAb detects endogenous levels of TNK1 protein only when the amplified gene product is phosphorylated at Tyr277.

Phospho-TNK1 (Tyr277) (D46E7) Rabbit mAb兔单抗能检测内源性基因扩增产物中Tyr277位点磷酸化的TNK1蛋白水平。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr277 of human TNK1 protein.


Western Blotting

Western Blotting

Western blot analysis of extracts from L-540 cells, untreated or pre-incubated with either a site-specific phosphorylated peptide to block the signal or a site-specific non-phosphorylated peptide, using Phospho-TNK1 (Tyr277) (D46E7) Rabbit mAb. L-540 cells express a 58 kDa TNK1-C17orf61 fusion protein containing 466 amino acids from the amino terminus of TNK1 [Gu, T.L. et al. (2010) Leukemia].western blot方法检测细胞提取物:未处理和与能阻断信号传递的位点特异性磷酸化肽段、位点特异性非磷酸化肽段提前孵育的L-540细胞。使用的抗体是Phospho-TNK1 (Tyr277) (D46E7) Rabbit mAb。L-540细胞表达58 kDa TNK1-C17和f61融合蛋白,TNK1胺端包含466个氨基酸[Gu, T.L. et al. (2010) Leukemia]。


Tyrosine kinase non-receptor 1 (TNK1) is related to the Ack1 (TNK2) non-receptor kinase that binds cdc42 and inhibits GTPase activity of this cell cycle regulator. TNK1 is broadly expressed in embryogenic tissues and leukemia cell lines, but is restricted to select adult tissues (1). TNK1 is a putative 72 kDa protein comprised of an N-terminal kinase domain, a central SH3 domain and a proline-rich tail. Interaction with PLCγ in vitro indicates a possible role in phospholipid signal transduction pathways (2). Though the exact mechanism is currently unclear, active TNK1 may play a role in regulating cell death by preventing TNF-α-induced NF-κB transactivation (3).

酪氨酸激酶非受体1(TNK1)与Ack1 (TNK2)非受体激酶相关,能够结合cdc42并抑制该细胞周期调节子的GTP酶活性 。TNK1广泛表达于胚胎发生组织和白血病细胞系中,但是在成人组织中的表达有所限制(1)。TNK1蛋白的大小大约是72 kDa,由N端激酶结构域、中心SH3结构域和一个富含脯氨酸的尾部区域组成(2)。尽管确切的发生机制目前尚不清楚,但是活化的TNK1蛋白可能会通过抑制TNF-α诱导的NF-κB转录激活而调节细胞死亡(3)。

Phosphorylation of TNK1 on Tyr277 was identified at Cell Signaling Technology (CST) using PhosphoScan®, CST's LC-MS/MS platform for phosphorylation site discovery (4) and also reported independently in another publication using MS technology (5). Phosphorylation of TNK1 at Tyr277 was observed in select carcinoma cell lines and in tumors. A constitutively active, 58 kDa truncated TNK1 kinase resulting from fusion between the TNK1 and C17orf61 genes is seen in some cells (5). For additional information, visit PhosphoSitePlus™, CST's modification site knowledgebase, at www.phosphosite.org.

TNK1的Tyr277位点的磷酸化由Cell Signaling Technology (CST)发现,使用的技术是PhosphoScan®——这是我们用于发现磷酸化位点的LC-MS/MS平台(4),同时也利用这项MS技术独立发表了文章(5)。在某些癌细胞系和肿瘤中观察到TNK1的Tyr277位点的磷酸化。在一些细胞中还观察到TNK1与C17orf61基因的融合导致截断了的TNK1激酶具有组成性活性(5)。更多信息详见PhosphoSitePlus™——CST的修饰位点信息库,网址是www.phosphosite.org.

  1. Hoehn, G.T. et al. (1996) Oncogene 12, 903-13.
  2. Felschow, D.M. et al. (2000) Biochem Biophys Res Commun 273, 294-301.
  3. Azoitei, N. et al. (2007) Oncogene 26, 6536-45.
  4. Rush, J. et al. (2005) Nat Biotechnol 23, 94-101.
  5. Gu, T.L. et al. (2010) Leukemia 24, 861-5.

Application References

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