Cell Signaling Technology

Product Pathways - NF-kB Signaling

IRF-8 (D20D8) Rabbit mAb #5628

sc-6058  

No. Size Price
5628S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
5628 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse, Endogenous 50 Rabbit IgG
IP 1:50
ChIP 1:25

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, ChIP=Chromatin IP,

Homology

Species predicted to react based on 100% sequence homology: Rat, Monkey, Xenopus, Bovine,

Specificity / Sensitivity

IRF-8 (D20D8) Rabbit mAb detect endogenous levels of total IRF-8 protein. An unknown background band is detected at 80 kDa in some cell lines.

IRF-8 (D20D8) Rabbit mAb能够检测内源IRF-8蛋白总体水平。在一些细胞系中,一个未知背景的80 kDa条带也能被检测到。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly65 of human IRF-8 protein.

此单克隆抗体是通过合成人源对应的IRF-8 Gly65位点周围的肽段来免疫动物而获得。

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 THP-1 and either 20 μl of IRF-8 (D20D8) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human GPR18 promoter primers, human MS4A7 promoter primers, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.染色质免疫共沉淀。 THP-1细胞培养至4 x 106,然后用SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003进行免疫沉淀实验,所用的抗体为20 μl IRF-8 (D20D8) Rabbit mAb或2 μl of Normal Rabbit IgG #2729,本实验中用 human GPR18 promoter primers, human MS4A7 promoter primers和 SimpleChIP® Human α Satellite Repeat Primers #4486引物对富集的DNA做real-time PCR。每个样本中沉淀的DNA量定义为相对信号与输入的总染色质相比的数值。

Western Blotting

Western Blotting

Western blot analysis of extracts from Raji and Jurkat cells, untreated or treated with Human Interferon-α1 (hIFN-α1) #8927 (10 ng/ml; overnight) using IRF-8 (D20D8) Rabbit mAb.Western免疫印迹。用IRF-8 (D20D8) Rabbit mAb研究未经处理的和经hIFN-α1 #8927 (10 ng/ml)处理过夜的Raji 和 Jurkat细胞的细胞提取液。

Western Blotting

Western Blotting

Western blot analysis of extracts from THP-1, RL7, and A20 cell lines using IRF-8 (D20D8) Rabbit mAb.Western免疫印迹。用IRF-8 (D20D8) Rabbit mAb研究THP-1, RL7 和 A20细胞系的细胞提取液。

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, mock transfected or transfected with human and mouse IRF-8 constructs, using IRF-8 (D20D8) Rabbit mAb.Western免疫印迹。用IRF-8 (D20D8) Rabbit mAb研究对照转染的和转染了人和小鼠IRF-8质粒的293T细胞的细胞提取液。

Background

Interferon regulatory factors (IRFs) comprise a family of transcription factors that function within the Jak/Stat pathway to regulate interferon (IFN) and IFN-inducible gene expression in response to viral infection (1). IRFs play an important role in pathogen defense, autoimmunity, lymphocyte development, cell growth, and susceptibility to transformation. The IRF family includes nine members: IRF-1, IRF-2, ISGF3γ/p48, IRF-3, IRF-4 (Pip/LSIRF/ICSAT), IRF-5, IRF-6, IRF-7, and IRF-8/ICSBP. All IRF proteins share homology in their amino-terminal DNA-binding domains. IRF family members regulate transcription through interactions with proteins that share similar DNA-binding motifs, such as IFN-stimulated response elements (ISRE), IFN consensus sequences (ICS) and IFN regulatory elements (IRF-E) (2).

干扰素调控因子(IRFs) 包含一个家族的转录调控因子,这个家族主要是在 Jak/Stat信号通路中起作用,在受到病毒的感染后能够调控干扰素(IFN)和干扰素诱导的基因表达(1)。IRFs在抵抗病原菌、自身免疫、淋巴细胞发育、细胞生长和易感性的转变等过程中发挥了重要的作用。IRF家族包含9个成员: IRF-1, IRF-2, ISGF3γ/p48, IRF-3, IRF-4 (Pip/LSIRF/ICSAT), IRF-5, IRF-6, IRF-7和IRF-8/ICSBP。所有的IRF蛋白在N-末端的DNA-结合区域都有同源性。IRF家族成员通过与具有相似的DNA-结合区域的蛋白的反应而调控转录,例如IFN刺激的应答原件(ISRE), IFN 保守基序 (ICS) 和IFN 调控元件(IRF-E) (2)。

IRF-8/ICSCP is expressed predominately in hematopoietic cells and is further increased upon treatment with interferon (3,4). IRF-8 can function as a transcription repressor of ICS-containing promoters (4). Expression of IRF-8 can lead to the down-regulation of the anti-apoptotic protein Bcl-2 (5). Originally described as being induced by IFN-γ, IRF-8 expression is also elevated by IRF-α as well as IL-12 in NK and T cells (6). IRF-8 deficient mice have enhanced susceptibility to various pathogens and impaired production of interferons, as well as deregulated hematopoiesis that resembles chronic myelogenous leukemia (7,8). IRF-8 also regulates bone metabolism by suppressing osteoclast formation (9). IRF-8/ICSCP主要是在造血细胞中表达,在受到干扰素的处理后而升高表达(3,4)。IRF-8 能作为含有ICS的启动子的转录抑制因子(4)。IRF-8的表达导致了抗凋亡蛋白Bcl-2的下调(5)。最初报道描述IRF-8表达被IFN-γ诱导, 在NK和T细胞中IRF-8的表达也能被IRF-α和IL-12诱导升高(6)。IRF-8缺失的小鼠对多种病原菌的易感性增强,干扰素的产生降低,类似于慢性骨髓性白血病对造血作用失去控制(7,8)。IRF-8也能通过抑制破骨细胞的形成而调控骨的新陈代谢(9)。

  1. Taniguchi, T. et al. (2001) Annu Rev Immunol 19, 623-55.
  2. Honda, K. and Taniguchi, T. (2006) Nat Rev Immunol 6, 644-58.
  3. Driggers, P.H. et al. (1990) Proc Natl Acad Sci U S A 87, 3743-7.
  4. Weisz, A. et al. (1992) J Biol Chem 267, 25589-96.
  5. Burchert, A. et al. (2004) Blood 103, 3480-9.
  6. Lehtonen, A. et al. (2003) Cytokine 24, 81-90.
  7. Holtschke, T. et al. (1996) Cell 87, 307-17.
  8. Fehr, T. et al. (1997) J Exp Med 185, 921-31.
  9. Zhao, B. et al. (2009) Nat Med 15, 1066-71.

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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