Cell Signaling Technology

Product Pathways - Apoptosis

Cleaved PARP (Asp214) (D64E10) XP® Rabbit mAb #5625

sc-7150   sc-8007  

No. Size Price
5625S 100 µl ( 10 western blots ) ¥4,050.00 现货查询 购买询价
5625T 20 µl ( 2 western blots ) ¥1,600.00 现货查询 购买询价
5625 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Monkey, Endogenous 89 Rabbit IgG
IP 1:100
IHC-P 1:50
F 1:400
IF-IC 1:400

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), F=Flow Cytometry, IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

Cleaved PARP (Asp214) (D64E10) XP® Rabbit mAb detects endogenous levels of the large fragment (89 kDa) of human PARP1 protein produced by caspase cleavage. The antibody does not recognize full length PARP1 or other PARP isoforms.

Cleaved PARP (Asp214) (D64E10) XP® Rabbit mAb兔单抗能够检测经caspase酶切的人源PARP1蛋白大片段(89 kDa)的内源性水平。该抗体不与全长PARP1或其它的PARP亚型发生交叉反应。

Source / Purification

Monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp214 in human PARP.




Confocal immunofluorescent analysis of HeLa cells, untreated (left) or treated with Staurosporine #9953 (right), using Cleaved PARP (Asp214) (D64E10) XP ® Rabbit mAb (green). Actin filament were labeled with DY-554 phalloidin. Blue pseudocolor = DRAQ5 ® #4084 (fluorescent DNA dye).

共聚焦免疫荧光分析HeLa细胞,未处理(左)和Staurosporine #9953(右)处理,使用抗体为Cleaved PARP (Asp214) (D64E10) XP ® Rabbit mAb兔单抗(绿色),DY-554 phalloidin(红色)标记肌动蛋白丝。蓝色伪彩=DRAQ5 ®#4084(荧光DNA染料)。

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, untreated or treated with Staurosporine #9953 (1 μM, 3 hr), Jurkat cells, untreated or etoposide-treated (25 μM, overnight), and THP-1 cells, untreated or cycloheximide-treated (CHX, 10 μg/ml, overnight) followed by treatment with TNF-α #8902 (20 ng/ml, 4 hr), using Cleaved PARP (Asp214) (D64E10) XP ® Rabbit mAb (upper), or total PARP Antibody #9542 (lower).

Western blot 检测各细胞提取物,Hela细胞,未处理 (-)或Staurosporine #9953 (1 μM, 3 hr)处理;Jurkat细胞,未处理 (-)或依托泊甙(25 μM, 过夜)处理;THP-1细胞,未处理 (-)或放线菌酮(CHX, 10 μg/ml,过夜)处理。再将所有样品用TNF-α #8902 (20 ng/ml, 4 hr)处理。使用抗体为Cleaved PARP (Asp214) (D64E10) XP ® Rabbit mAb兔单抗(上图)或总PARP Antibody #9542(下图)。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Jurkat cells, untreated (blue) or etoposide-treated (green), using Cleaved PARP (Asp214) (D64E10) XP® Rabbit mAb.

流式细胞术检测Jurkat细胞,未处理(蓝)和etoposide处理(绿),使用抗体为Cleaved PARP (Asp214) (D64E10) XP® Rabbit mAb兔单抗。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human tonsil using Cleaved PARP (Asp214) (D64E10) XP® Rabbit mAb.

免疫组织化学分析石蜡包埋的人结扁桃体切片,使用抗体为 Cleaved PARP (Asp214) (D64E10) XP® Rabbit mAb兔单抗。


PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress (1). This protein can be cleaved by many ICE-like caspases in vitro (2,3) and is one of the main cleavage targets of caspase-3 in vivo (4,5). In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa) (2,4). PARP helps cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis (6).

PARP,是核多聚(ADP-核糖) 聚合酶,分子量116kDa,参与环境胁迫应答中的DNA修复(1)。在体外该蛋白可被许多ICE样 caspases裂解(2,3),在体内是caspase-3裂解的主要靶蛋白之一(4,5)。在人体中PARP在位点Asp214 和 Gly215被裂解,裂解后PARR的N末端DNA 结合结构域(24 kDa)与C端催化结构域(89 kDa) 分离(2,4)。PARP协助细胞维持生存能力;PARP的裂解促进细胞崩解并可以作为细胞凋亡的标记物(6)。

  1. Satoh, M.S. and Lindahl, T. (1992) Nature 356, 356-358.
  2. Lazebnik, Y. A. et al. (1994) Nature 371, 346-347.
  3. Cohen, G.M. (1997) Biochem. J. 326, 1-16.
  4. Nicholson, D. W. et al. (1995) Nature 376, 37-43.
  5. Tewari, M. et al. (1995) Cell 81, 801-809.
  6. Oliver, F.J. et al. (1998) J. Biol. Chem. 273, 33533-33539.

Application References

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