Cell Signaling Technology

Product Pathways - Neuroscience

Phospho-Semaphorin 4B (Ser825) Antibody #5622


No. Size Price
5622S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
5622 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 105 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Phospho-Semaphorin 4B (Ser825) Antibody detects endogenous levels of semaphorin 4B protein only when phosphorylated at Ser825.

Phospho-Semaphorin 4B (Ser825) Antibody 兔多抗识别内源性的Ser825磷酸化的semaphorin 4B蛋白。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser825 of human semaphorin 4B protein. Antibodies are purified by protein A and peptide affinity chromatography.

该多克隆抗体由合成的人类semaphorin 4B蛋白Ser825位点附近磷酸化肽段免疫动物而制成。该抗体使用蛋白A和多肽亲和层析纯化而得。

Western Blotting

Western Blotting

Western blot analysis of extracts from MKN-45 cells, untreated or SU11274-treated (1 μM for 2 hr), using Phospho-Semaphorin 4B (Ser825) Antibody (upper) and Semaphorin 4B Antibody #3355 (lower).

Western blot分析MKN-45细胞(未处理,或1uM SU11274处理2小时),使用的抗体是Phospho-Semaphorin 4B (Ser825) Antibody 兔多抗(上图)或Semaphorin 4B Antibody 兔多抗#3355(下图)。


The semaphorin family of proteins is involved in axon guidance, cell migration, angiogenesis, and immune response. Plexins and neuropilins bind with high affinity to semaphorins to mediate their functions. Semaphorins are divided into seven classes of secreted or membrane-bound proteins. Members of Class 4 semaphorins include 4A through 4G; semaphorin 4B is a membrane-associated protein (1,2). Semaphorin 4B binds to the CLCP1 receptor and regulates cell motility (3). Furthermore, semaphorin 4B, like many other semaphorins, has a PDZ domain-binding motif at the carboxy terminus that interacts with PSD95 and localizes to the post-synaptic membrane (4).The phosphorylation site at Ser825 was identified at Cell Signaling Technology using PTMScan® Technology, a proprietary proteomic method for post-translational modification site discovery (5).

Semaphorin家族的蛋白参与轴突导向,细胞迁移,血管生成和免疫反应。Semaphorin能与plexins和neuropilins高亲和力结合,调节它们的功能。Semaphorins可分为7类分泌或膜结合蛋白。第4类semaphorins成员包括4A-4G;Semaphorin 4B是一种膜相关蛋白(1,2)。Semaphorin 4B与CLCP1受体结合,调节细胞运动(3)。此外,semaphorin 4B,和许多其它semaphorins一样,有一个PDZ结构域结合模体在羧基端,可与PSD95相互作用,定位于突触后膜(4)。Ser825磷酸化位点由CST公司使用PTMScan®技术(一种自有的针对转录后修饰的蛋白组学方法)发现(5)。

  1. Pasterkamp, R.J. and Kolodkin, A.L. (2003) Curr Opin Neurobiol 13, 79-89.
  2. Kruger, R.P. et al. (2005) Nat Rev Mol Cell Biol 6, 789-800.
  3. Nagai, H. et al. (2007) Oncogene 26, 4025-31.
  4. Burkhardt, C. et al. (2005) FEBS Lett 579, 3821-8.
  5. Moritz, A. et al. (2010) Sci Signal 3, ra64.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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