Cell Signaling Technology

Product Pathways - Screening Technologies

K63-linkage Specific Polyubiquitin (D7A11) Rabbit mAb #5621

K-63   K63   K63-linked   K63-ub   ub   ubiquitin  

No. Size Price
5621S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
5621 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 All Species Expected, Endogenous Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

K63-linkage Specific Polyubiquitin (D7A11)兔单抗识别Lys63位偶联的多泛素化链,这一抗体与单泛素和其他残基偶联的多泛素链无交叉反应。

Source / Purification

这一单抗是通过合成与人双泛素链的Lys63位分枝序列一致的肽段,免疫动物获得。 Western blot analysis of extracts from HeLa cells, untreated or treated with the proteasome inhibitor MG132 (10 µM for 6 hours), using K63-linkage Specific Polyubiquitin (D7A11) Rabbit mAb and Ubiquitin Antibody #3933 (lower). 使用K63偶联多泛素(D7A11)兔单抗和泛素抗体#3933(下)对未经处理或经蛋白酶体抑制剂MG132 (10 µM,6小时)处理的Hela细胞裂解物进行免疫印迹分析的结果。 Western blot analysis of seven distinct recombinant polyubiquitin chains using K48-linkage Specific Polyubiquitin Antibody(D7A11) (upper) and Ubiquitin (P4D1) Mouse mAb #3933 (lower). 使用K63偶联多泛素(D7A11)抗体(上)和泛素(P4D1)小鼠单克隆抗体#3933(下)对七个不同的重组泛素链(每孔加样300ng)进行Western blot分析的结果。 Western blot analysis comparing the titration of recombinant monoubiquitin, K48-linked polyubiquitin and K63-linked polyubiquitin using K63-linkage Specific Polyubiquitin (D7A11) Rabbit mAb (upper) and Ubiquitin Antibody #3933 (lower). 使用K63偶联多泛素(D7A11)兔单抗(上)和泛素(P4D1)小鼠单克隆抗体#3933(下)对重组单泛素、重组K48偶联多泛素和K63偶联多泛素进行滴定对比的免疫印迹分析结果。 Western blot analysis of various cell lines using K63-linkage Specific Polyubiquitin (D7A11) Rabbit mAb. 使用K63偶联多泛素(D7A11)兔单抗对不同细胞裂解物进行免疫印迹分析的结果。 Western blot analysis of extracts from HeLa cells using K63-linkage Specific Polyubiquitin (D7A11) Rabbit mAb, untreated or following antibody pre-incubation with either K63 ubiquitinylated branched peptide to block the signal or a linear peptide surrounding K63 of ubiquitin that cannot block the signal. 使用K63偶联多泛素(D7A11)兔单抗,未经处理或通过和能够封闭免疫反应信号的K63泛素化分枝多肽或不能封闭信号的K63位泛素周围序列的线性化多肽孵育后,对Hela细胞裂解物进行免疫印迹分析的结果。

Western Blotting

Western Blotting

Western blot analysis comparing the titration of recombinant monoubiquitin, K48-linked polyubiquitin and K63-linked polyubiquitin using K63-linkage Specific Polyubiquitin (D7A11) Rabbit mAb (upper) and Ubiquitin Antibody #3933 (lower).

Western Blotting

Western Blotting

Western blot analysis of seven distinct recombinant polyubiquitin chains (300 ng each) using K63-linkage Specific Polyubiquitin (D7A11) Rabbit mAb (upper) and Ubiquitin Antibody #3933 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, untreated or treated with the proteasome inhibitor MG132 (10 µM for 6 hours), using K63-linkage Specific Polyubiquitin (D7A11) Rabbit mAb and Ubiquitin Antibody #3933 (lower).

Western Blotting

Western Blotting

Western blot analysis of various cell lines using K63-linkage Specific Polyubiquitin (D7A11) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells using K63-linkage Specific Polyubiquitin (D7A11) Rabbit mAb, untreated or following antibody pre-incubation with either K63 ubiquitinylated branched peptide to block the signal or a linear peptide surrounding K63 of ubiquitin that cannot block the signal.

Background

泛素是一个在泛素——蛋白酶体途径中发挥重要作用的保守多肽。泛素能够通过泛素转移过程被共价修饰于多个细胞蛋白,导致蛋白通过26S蛋白酶体降解。三个泛素化酶但与泛素偶联过程。泛素首先与活化酶E1形成硫酯键复合体而被激活,然后被转移到泛素载体蛋白E2,然后由E2转移到E3并连接到靶蛋白赖氨酸的epsilon位氨基上(1-3)。泛素——蛋白酶体途径参与多种生物过程和疾病发生,多种信号通路蛋白如IκB、p53、cdc25a 和Bcl-2都收到泛素——蛋白酶体途径调节,影响细胞周期进程、分化、细胞应激和凋亡等过程(4-7)。

在底物蛋白泛素化修饰过程中,最常见的连接位点为泛素的7个赖氨酸残基(Lys6, Lys11, Lys27, Lys29, Lys33, Lys48 and Lys63)。 多泛素链的形成是通过一个泛素的赖氨酸残基又连接到其他泛素羧基端的甘氨酸残基。在不同lysine残基进行偶联的多泛素化组装介导着不同的功能(8)。K63位偶联的多泛素化主要调节一些非蛋白裂解过程,如蛋白质转运、激酶/磷酸酶活性、DNA损伤调控等过程,对于癌细胞存活和癌症发展过程至关重要(9,10)。

  1. Ciechanover, A. (1998) EMBO J 17, 7151-60.
  2. Hochstrasser, M. (2000) Nat Cell Biol 2, E153-7.
  3. Hochstrasser, M. (2000) Science 289, 563-4.
  4. Bernardi, R. et al. (2000) Oncogene 19, 2447-54.
  5. Aberle, H. et al. (1997) EMBO J 16, 3797-804.
  6. Salomoni, P. and Pandolfi, P.P. (2002) Nat Cell Biol 4, E152-3.
  7. Jesenberger, V. and Jentsch, S. (2002) Nat Rev Mol Cell Biol 3, 112-21.
  8. Komander, D. (2009) Biochem Soc Trans 37, 937-53.
  9. Chen, Z.J. and Sun, L.J. (2009) Mol Cell 33, 275-86.
  10. Yang, W.L. et al. (2010) Oncogene 29, 4493-503.

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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