Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

Phospho-ARHGAP42 (Tyr376) (D45E9) Rabbit mAb #5617

No. Size Price
5617S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
5617 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Monkey, Endogenous 125 Rabbit IgG
IP 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,


Species predicted to react based on 100% sequence homology: Rat,

Specificity / Sensitivity

Phospho-ARHGAP42 (Tyr376) (D45E9) Rabbit mAb detects endogenous levels of ARHGAP42 only when phosphorylated at Tyr376. Phospho-ARHGAP42 (Tyr376) (D45E9) Rabbit mAb检测仅在Tyr376位点磷酸化的内源性ARHGAP42蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr376 of human ARHGAP42 protein. 通过人工合成人源ARHGAP42蛋白Tyr376位点周围相应的磷酸化片段去免疫动物从而制备单克隆抗体。

Western Blotting

Western Blotting

Western blot analysis of extracts from COS-7 cells using Phospho-ARHGAP42 (Tyr376) (D45E9) Rabbit mAb. The phospho-specificity of the antibody was verified by preincubating the antibody with no peptide, phosphopeptide, or the corresponding non-phosphopeptide prior to incubating the membrane. 使用Phospho-ARHGAP42 (Tyr376) (D45E9) Rabbit mAb,免疫印迹(Western Blot)分析COS-7细胞系Phospho-ARHGAP42 (Tyr376)蛋白水平。该抗体的磷酸化特异性在孵化膜之前通过预先孵育no peptide、phosphopeptide或相应的non-phosphopeptide确定。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Phospho-ARHGAP42 (Tyr376) (D45E9) Rabbit mAb. 使用Phospho-ARHGAP42 (Tyr376) (D45E9) Rabbit mAb,免疫印迹(Western Blot)分析不同细胞系Phospho-ARHGAP42 (Tyr376)蛋白水平。


ARHGAP family members activate Rho GTPase proteins (1). Mutations of ARHGAP family genes lead to carcinogenesis through the dysregulation of Rho/Rac/Cdc42-like GTPases (2). Little is known about the biological function of ARHGAP42, a member of the ARHGAP family. ARHGAP42 contains a RhoGAP domain in the middle of its sequence, an SH3 binding domain at the C-terminal end of the protein sequence, as well as a BAR domain and a PH domain in the N-terminal region of the sequence, which indicate that the protein may function as GTPase activator and cytoskeletal adaptor. ARHGAP家族成员激活Rho GTPase蛋白质(1)。ARHGAP家族基因的突变通过Rho/Rac/Cdc42-like GTPases异常调节导致致癌(2)。很少了解关于ARHGAP42蛋白的生理功能,其是ARHGAP家族成员之一。ARHGAP42蛋白包含一个蛋白序列中间的RhoGAP功能域、蛋白序列羧基末端SH3结合域、BAR结构域和序列氨基端PH功能域,这些表明该蛋白可能作为GTPase 激活因子和细胞骨架调节因子。

  1. Katoh, M. and Katoh, M. (2004) Int J Mol Med 14, 333-8.
  2. Katoh, M. and Katoh, M. (2004) Int J Oncol 25, 1201-6.

Application References

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