Cell Signaling Technology

Product Pathways - Metabolism

Phospho-IRS-1 (Ser318) (D51C3) Rabbit mAb #5610


No. Size Price
5610S 100 µl ( 10 western blots ) ¥4,050.00 现货查询 购买询价 防伪查询
5610 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse, Endogenous 180 Rabbit IgG
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,


Species predicted to react based on 100% sequence homology: Rat,

Specificity / Sensitivity

Phospho-IRS-1 (Ser318) (D51C3) Rabbit mAb recognizes endogenous levels of IRS-1 protein only when phosphorylated at Ser318. Note: Ser318 is the mouse residue; the corresponding human residue is Ser323. The antibody cross reacts with a inducible nonspecific band at around 57 kDa.

Phospho-IRS-1 (Ser318) (D51C3) Rabbit mAb用于检测内源性在318位丝氨酸位点磷酸化的IRS-1蛋白水平。请注意:Ser318指的是小鼠蛋白位点,人的对应位点为Ser323位。抗体会在57 kDa处有一个非特异性的诱导性交互反应条带。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser318 of mouse IRS-1 protein.


Western Blotting

Western Blotting

Western blot analysis of extracts from serum-starved C2C12 cells, untreated or insulin-treated (150 nM for 5 min.), using Phospho-IRS-1 (Ser318) (D51C3) Rabbit mAb (upper), or IRS-1 Antibody #3407 (lower).

Wetern blot检测血清饥饿的C2C12细胞提取物,未处理或用胰岛素(150 nM,5min)处理,所用抗体为 Phospho-IRS-1 (Ser318) (D51C3) Rabbit mAb兔单抗(上)和IRS-1 Antibody #3407 (下)。


Insulin receptor substrate 1 (IRS-1) is one of the major substrates of the insulin receptor kinase (1). IRS-1 contains multiple tyrosine phosphorylation motifs that serve as docking sites for SH2-domain containing proteins that mediate the metabolic and growth-promoting functions of insulin (2-4). IRS-1 also contains over 30 potential serine/threonine phosphorylation sites. Ser307 of IRS-1 is phosphorylated by JNK (5) and IKK (6) while Ser789 is phosphorylated by SIK-2, a member of the AMPK family (7). The PKC and mTOR pathways mediate phosphorylation of IRS-1 at Ser612 and Ser636/639, respectively (8,9). Phosphorylation of IRS-1 at Ser1101 is mediated by PKCθ and results in an inhibition of insulin signaling in the cell, suggesting a potential mechanism for insulin resistance in some models of obesity (10).

胰岛素受体底物1(IRS-1)是胰岛素受体激酶的一类主要底物(1)。 IRS-1包含多个酪氨酸磷酸化位点,可以作为含SH2结构域蛋白的停靠位点,这些蛋白调控着胰岛素的代谢和促生长功能(2-4) 。IRS-1还包含着起过30个潜在的丝氨酸/苏氨酸磷酸化位点,307位丝氨酸可以被JNK(5)和IKK(6)磷酸化,789位的丝氨酸可以被SIK-2(是AMPK家族成员)磷酸化(7)。PKC和mTOR途径可以分别磷酸化IRS-1的612位丝氨酸和636/639位丝氨酸(8,9)。PKCθ可以介导IRS-2在1101位丝氨酸位的磷酸化,此位点的磷酸化会导致细胞胰岛素信号通路的抑制,因此它可能在某些肥胖模型的胰岛素抵抗中起着某些作用(10)。

PKC phosphorylates mouse IRS-1 at Ser318 (human Ser323) by insulin receptor activation or by other stimulation such as TPA, IL-6, retinoic acid treatment (11-14) . The phosphorylation at Ser318 acts as a negative feedback signal to down regulates of insulin effect (14-16).


  1. Sun, X.J. et al. (1991) Nature 352, 73-77.
  2. Sun, X.J. et al. (1992) J. Biol. Chem. 267, 22662-22672.
  3. Myers Jr., M.G. et al. (1993) Endocrinology 132, 1421-1430.
  4. Wang, L.M. et al. (1993) Science 261, 1591-1594.
  5. Rui, L. et al. (1997) J. Clin. Invest. 107, 181-189.
  6. Gao, Z. et al. (2002) J. Biol. Chem. 277, 48115-48121.
  7. Horike, N. et al. (2003) J. Biol. Chem. 278, 18440-18447.
  8. Ozes, O.N. et al. (2001) Proc. Natl. Acad. Sci. USA 98, 4640-4645.
  9. De Fea, K. and Ruth, R.A. (1997) Biochemistry 36, 12939-12947.
  10. Li, Y. et al. (2004) J. Biol. Chem. 279, 45304-45307.
  11. Greene, M.W. et al. (2004) Biochem J 378, 105-116.
  12. Weigert, C. et al. (2006) J Biol Chem 281, 7060-7067.
  13. del Rincón, S.V. et al. (2004) Oncogene 23, 9269-9279.
  14. Moeschel, K. et al. (2004) J Biol Chem 279, 25157-25163.
  15. Weigert, C. et al. (2005) J Biol Chem 280, 37393-37399.
  16. Hennige, A.M. et al. (2006) FASEB J 20, 1206-1208.

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