Cell Signaling Technology

Product Pathways - PI3K / Akt Signaling

Phospho-SGK1 (Ser78) (D36D11) Rabbit mAb #5599

No. Size Price
5599S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
5599 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 54 Rabbit IgG
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,


Species predicted to react based on 100% sequence homology: Mouse, Rat, Monkey,

Specificity / Sensitivity

Phospho-SGK1 (Ser78) (D36D11) Rabbit mAb detects endogeneous levels of SGK1 protein only when phosphorylated at Ser78. This antibody does not detect isoforms SGK2 or SGK3.

Phospho-SGK1 (Ser78) (D36D11) Rabbit mAb可以检测在Ser78位点磷酸化的内源性SGK1蛋白。此抗体不能检测SGK2 或SGK3亚型。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser78 of human SGK1 protein.


Western Blotting

Western Blotting

Western blot analysis of extracts from COS cells transfected with SGK1 using Phospho-SGK1 (Ser78) (D36D11) Rabbit mAb, untreated or following antibody pre-incubation with either a site-specific phosphorylated peptide to block the signal or a site-specific non-phosphorylated peptide that cannot block the signal.

Western blot分析人类SGK1 (+)转染的COS细胞提取物,所用抗体为Phospho-SGK1 (Ser78) (D36D11) Rabbit mAb兔单抗,未处理或使用位点特异性磷酸化肽段封闭信号或使用位点特异性的非磷酸化肽段不能封闭信号。

Western Blotting

Western Blotting

Western blot analysis of extracts from COS cells, untransfected (-) or transfected with human SGK1 (+), using Phospho-SGK1 (Ser78) (D36D11) (upper) or SGK1 Antibody #3272 (lower).

Western blot分析COS细胞提取物,未转染组(-)和采用人类SGK1 (+)转染组,所用抗体为Phospho-SGK1 (Ser78) (D36D11) (上) 或 SGK1 Antibody #3272 (下)。

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, untreated (-) or treated with H2O2 (4 mM for 15 min), using Phospho-SGK1 (Ser78) (D36D11) (upper) or SGK1 Antibody #3272 (lower).

Western blot分析Hela细胞提取物,未转染组(-)和H2O2 (4 mM for 15 min)转染组,所用抗体为Phospho-SGK1 (Ser78) (D36D11) (上) 或 SGK1 Antibody #3272 (下)。


Serum and glucocorticoid-inducible kinase (SGK) is a serine/threonine kinase closely related to Akt (1). SGK is rapidly induced in response to a variety of stimuli, including serum, glucocorticoid, follicle stimulating hormone, osmotic shock, and mineralocorticoids. SGK activation can be accomplished via HGF PI3K-dependent pathways and by integrin-mediated PI3K-independent pathways (2,3). Induction and activation of SGK has been implicated in activating the modulation of anti-apoptotic and cell cycle regulation (4-6). SGK also plays an important role in activating certain potassium, sodium, and chloride channels, suggesting its involvement in the regulation of processes such as cell survival, neuronal excitability, and renal sodium excretion (2). SGK is negatively regulated by ubiquitination and proteasome degradation (7).


The MAP kinase family member BMK1 interacts with and activates SGK by phosphorylation at serine 78 (6).MAP激酶家族成员BMK1可以通过在丝氨酸78位点的磷酸化结合并激活SGK蛋白(6)。

  1. Webster, M.K. et al. (1993) Mol Cell Biol 13, 2031-40.
  2. Kobayashi, T. and Cohen, P. (1999) Biochem J 339 ( Pt 2), 319-28.
  3. Park, J. et al. (1999) EMBO J 18, 3024-33.
  4. Brunet, A. et al. (2001) Mol Cell Biol 21, 952-65.
  5. Mikosz, C.A. et al. (2001) J Biol Chem 276, 16649-54.
  6. Hayashi, M. et al. (2001) J Biol Chem 276, 8631-4.
  7. Brickley, D.R. et al. (2002) J Biol Chem 277, 43064-70.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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