Cell Signaling Technology

Product Pathways - PI3K / Akt Signaling

Phospho-SGK1 (Ser78) (D36D11) Rabbit mAb #5599

No. Size Price
5599S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
5599 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 54 Rabbit IgG
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Homology

Species predicted to react based on 100% sequence homology: Mouse, Rat, Monkey,

Specificity / Sensitivity

Phospho-SGK1 (Ser78) (D36D11) Rabbit mAb detects endogeneous levels of SGK1 protein only when phosphorylated at Ser78. This antibody does not detect isoforms SGK2 or SGK3.

Phospho-SGK1 (Ser78) (D36D11) Rabbit mAb可以检测在Ser78位点磷酸化的内源性SGK1蛋白。此抗体不能检测SGK2 或SGK3亚型。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser78 of human SGK1 protein.

单克隆抗体是采用人源SGK1蛋白的Ser78周围残基相对应的合成肽段免疫动物生产的。

Western Blotting

Western Blotting

Western blot analysis of extracts from COS cells transfected with SGK1 using Phospho-SGK1 (Ser78) (D36D11) Rabbit mAb, untreated or following antibody pre-incubation with either a site-specific phosphorylated peptide to block the signal or a site-specific non-phosphorylated peptide that cannot block the signal.

Western blot分析人类SGK1 (+)转染的COS细胞提取物,所用抗体为Phospho-SGK1 (Ser78) (D36D11) Rabbit mAb兔单抗,未处理或使用位点特异性磷酸化肽段封闭信号或使用位点特异性的非磷酸化肽段不能封闭信号。

Western Blotting

Western Blotting

Western blot analysis of extracts from COS cells, untransfected (-) or transfected with human SGK1 (+), using Phospho-SGK1 (Ser78) (D36D11) (upper) or SGK1 Antibody #3272 (lower).

Western blot分析COS细胞提取物,未转染组(-)和采用人类SGK1 (+)转染组,所用抗体为Phospho-SGK1 (Ser78) (D36D11) (上) 或 SGK1 Antibody #3272 (下)。

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, untreated (-) or treated with H2O2 (4 mM for 15 min), using Phospho-SGK1 (Ser78) (D36D11) (upper) or SGK1 Antibody #3272 (lower).

Western blot分析Hela细胞提取物,未转染组(-)和H2O2 (4 mM for 15 min)转染组,所用抗体为Phospho-SGK1 (Ser78) (D36D11) (上) 或 SGK1 Antibody #3272 (下)。

Background

Serum and glucocorticoid-inducible kinase (SGK) is a serine/threonine kinase closely related to Akt (1). SGK is rapidly induced in response to a variety of stimuli, including serum, glucocorticoid, follicle stimulating hormone, osmotic shock, and mineralocorticoids. SGK activation can be accomplished via HGF PI3K-dependent pathways and by integrin-mediated PI3K-independent pathways (2,3). Induction and activation of SGK has been implicated in activating the modulation of anti-apoptotic and cell cycle regulation (4-6). SGK also plays an important role in activating certain potassium, sodium, and chloride channels, suggesting its involvement in the regulation of processes such as cell survival, neuronal excitability, and renal sodium excretion (2). SGK is negatively regulated by ubiquitination and proteasome degradation (7).

血清和糖皮质激素诱导激酶(SGK)是一个丝氨酸/苏氨酸激酶,与Akt紧密联系(1)。SGK可以被多种刺激包括血清,糖皮质激素,卵泡刺激素,渗透压冲击和盐皮质激素迅速诱导。SGK可以被肝细胞生长因子HGF刺激的PI3K依赖的通路和整合素介导的PI3K非依赖的通路激活(2,3)。SGK的诱导和激活参与激活细胞抗凋亡和细胞周期调控(4-6)。SGK在钾,钠,氯离子通道激活中也起重要作用,表明其参与调控细胞的存活,神经元的兴奋,肾钠排泄等过程(2)。SGK被泛素化和蛋白酶体降解等过程负向调控(7)。

The MAP kinase family member BMK1 interacts with and activates SGK by phosphorylation at serine 78 (6).MAP激酶家族成员BMK1可以通过在丝氨酸78位点的磷酸化结合并激活SGK蛋白(6)。

  1. Webster, M.K. et al. (1993) Mol Cell Biol 13, 2031-40.
  2. Kobayashi, T. and Cohen, P. (1999) Biochem J 339 ( Pt 2), 319-28.
  3. Park, J. et al. (1999) EMBO J 18, 3024-33.
  4. Brunet, A. et al. (2001) Mol Cell Biol 21, 952-65.
  5. Mikosz, C.A. et al. (2001) J Biol Chem 276, 16649-54.
  6. Hayashi, M. et al. (2001) J Biol Chem 276, 8631-4.
  7. Brickley, D.R. et al. (2002) J Biol Chem 277, 43064-70.

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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