Cell Signaling Technology

Product Pathways - Adhesion

Syndecan 1 (D4Y7H) Rabbit mAb (PE Conjugate) #55987

No. Size Price
55987S 100 µl ( 50 tests ) ¥3,876.00 现货查询 购买询价
55987 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
F 1:50 Human, Endogenous Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: F=Flow Cytometry,

Specificity / Sensitivity

Syndecan 1 (D4Y7H) Rabbit mAb (PE Conjugate) recognizes endogenous levels of multimeric forms of syndecan 1 protein. The unconjugated antibody cross-reacts with proteins of unknown origin between 46-60 kDa in some cell lines by western blot analysis.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala294 of human syndecan 1 protein.

Description

This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. This antibody is expected to exhibit the same species cross-reactivity as the unconjugated Syndecan 1 (D4Y7H) Rabbit mAb #12922.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of 293T cells (blue) and U266 cells (green) using Syndecan 1 (D4Y7H) Rabbit mAb (PE Conjugate).

Background

Syndecans are a family of type 1 transmembrane heparan sulphate proteoglycans comprising 4 members in mammals (SDC-1 to -4) (1) encoded by four syndecan genes. Syndecans are involved in embryonic development, tumorigenesis, and angiogenesis (2). The extracellular domain harbors attachment sites for heparan sulfate and chondroitin sulfate chains, facilitating interaction with an array of proteins including a plethora of growth factors. In addition, the hydrophobic C-terminal intracellular domain can interact with proteins containing a PDZ domain (2). These interactions place syndecans as important integrators of membrane signaling (3). Syndecans undergo proteolytic cleavage causing the release of their extracellular domain (shedding), converting the membrane-bound proteins into soluble molecular effectors (4).

  1. Couchman, J.R. (2003) Nat Rev Mol Cell Biol 4, 926-37.
  2. Multhaupt, H.A. et al. (2009) J Physiol Pharmacol 60 Suppl 4, 31-8.
  3. Zimmermann, P. and David, G. (1999) FASEB J 13 Suppl, S91-S100.
  4. Manon-Jensen, T. et al. (2010) FEBS J 277, 3876-89.

Application References

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Protocols

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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