Cell Signaling Technology

Product Pathways - PI3K / Akt Signaling

Phospho-Tuberin/TSC2 (Ser1387) Antibody #5584

Hamartin  

No. Size Price
5584S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
5584T 20 µl ( 2 western blots ) ¥1,500.00 现货查询 购买询价
5584 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 200 Rabbit
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

Phospho-Tuberin/TSC2 (Ser1387) Antibody detects endogenous levels of tuberin protein only when phosphorylated at Ser1387. This antibody also cross-reacts with an unidentified 140 kD protein.

Phospho-Tuberin/TSC2 (Ser1387) Antibody可以检测出在ser1387位点磷酸化的内源性总马铃薯球蛋白(Tuberin),此抗体可以与140kD的未知蛋白发生交叉反应。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser1387 of human tuberin protein. Antibodies are purified by protein A and peptide affinity chromatography.

多克隆抗体是采用人类马铃薯球蛋白(Tuberin)Ser1387周围残基相对应的合成磷酸肽段免疫动物生产的,抗体采用蛋白A和肽亲和层析法纯化的。

Western Blotting

Western Blotting

Western blot analysis of extracts from SH-SY5Y cells, treated with AICAR #9944 (2 mM for 30 minutes), using Phospho-Tuberin/TSC2 (Ser1387) Antibody. The phospho-specificity of the antibody was verified by treating the membrane with (+) or without (-) calf intestinal phosphatase (CIP) after western transfer.Western blot 分析SH-SY5Y细胞提取物,未处理组和AICAR #9944 (2 mM for 30 minutes)处理组,所用抗体为Phospho-Tuberin/TSC2 (Ser1387) Antibody。抗体的磷酸特异性是由western 转膜后膜经过或不经小牛肠激酶(CIP)处理来验证的。

Western Blotting

Western Blotting

Western blot analysis of extracts from SH-SY5Y cells, untreated or treated with AICAR #9944 (2 mM for 30 minutes), using Phospho-Tuberin/TSC2 (Ser1387) Antibody (upper) and Tuberin/TSC2 (D93F12) XP® Rabbit mAb #4308 (lower).Western blot 分析SH-SY5Y细胞提取物,未处理组和AICAR #9944 (2 mM for 30 minutes)处理组,所用抗体为Phospho-Tuberin/TSC2 (Ser1387) Antibody (上) 和Tuberin/TSC2 (D93F12) XP™ Rabbit mAb #4308 (下).

Background

Tuberin is a product of the TSC2 tumor suppressor gene and an important regulator of cell proliferation and tumor development (1). Mutations in either TSC2 or the related TSC1 (hamartin) gene cause tuberous sclerosis complex (TSC), an autosomal dominant disorder characterized by development of multiple, widespread non-malignant tumors (2). Tuberin is directly phosphorylated at Thr1462 by Akt/PKB (3). Phosphorylation at Thr1462 and Tyr1571 regulates tuberin-hamartin complexes and tuberin activity (3-5). In addition, tuberin inhibits the mammalian target of rapamycin (mTOR), which promotes inhibition of p70 S6 kinase, activation of eukaryotic initiation factor 4E binding protein 1 (4E-BP1, an inhibitor of translation initiation), and eventual inhibition of translation (3,6,7).Phosphorylation of tuberin by AMPK at Ser1387 is necessary for cell size control in response to energy deprivation and protects from apoptosis (8). Furthermore, phosphorylation at Ser1387 primes phosphorylation by GSK3 of upstream sites (Ser1383, Ser1379 and Ser1375), integrating Wnt signaling (9).

马铃薯球蛋白(Tuberin)是TSC2肿瘤抑制基因的产物,是细胞增殖和肿瘤发展的重要调节因子(1)。TSC2或与之相关的TSC1(hamartin)的突变可以引起结节硬化复合体TSC(tuberous sclerosis complex),是一种常染色体显性遗传病,其特点为发生多种广泛的非恶性肿瘤(2)。Tuberin可以在Thr1462位点直接被Akt/PKB 磷酸化(3)。 在Thr1462 和 Tyr1571位点的磷酸化可以调节tuberin-hamartin复合体和tuberin的活性(3-5)。另外,tuberin可以抑制mTOR(mammalian target of rapamycin), 进而推动p70 S6激酶的抑制和真核细胞抑制因子4E结合蛋白(4E-BP1,一种转录起始抑制因子)的活性,并最终抑制翻译(3,6,7)。Tuberin被AMPK 在Ser1387位点磷酸化是使细胞在能量供应减少情况下对细胞大小控制以保护细胞不凋亡(8)。 另外,在Ser1387磷酸化为在上游位点(Ser1383, Ser1379 and Ser1375)被GSK3磷酸化做好准备,参与Wnt信号通路(9)。

  1. Soucek, T. et al. (1998) Proc Natl Acad Sci U S A 95, 15653-8.
  2. Sparagana, S.P. and Roach, E.S. (2000) Curr Opin Neurol 13, 115-9.
  3. Manning, B.D. et al. (2002) Mol Cell 10, 151-62.
  4. Aicher, L.D. et al. (2001) J Biol Chem 276, 21017-21.
  5. Dan, H.C. et al. (2002) J Biol Chem 277, 35364-70.
  6. Goncharova, E.A. et al. (2002) J Biol Chem 277, 30958-67.
  7. Inoki, K. et al. (2002) Nat Cell Biol 4, 648-57.
  8. Inoki, K. et al. (2003) Cell 115, 577-590.
  9. Inoki, K. et al. (2006) Cell 126, 955-968.

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