Cell Signaling Technology

Product Pathways - PI3K / Akt Signaling

Phospho-GSK-3β (Ser9) (D85E12) XP® Rabbit mAb #5558

GSK-3beta   sc-11757  

No. Size Price
5558S 100 µl ( 10 western blots ) ¥3,990.00 现货查询 购买询价
5558T 20 µl ( 2 western blots ) ¥1,600.00 现货查询 购买询价
5558 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Hamster, Endogenous 46 Rabbit IgG
IP 1:50
F 1:200
IF-IC 1:400

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, F=Flow Cytometry, IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

Phospho-GSK-3β (Ser9) (D85E12) XP® Rabbit mAb detects endogenous levels of GSK-3β only when phosphorylated at Ser9. This antibody reacts with denatured components of bovine serum, including BSA.

Phospho-GSK-3β (Ser9) D85E12) XP ® Rabbit mAb兔单抗可以检测出在ser9位点磷酸化的内源性总GSK-3β蛋白水平,此抗体可以与胎牛血清中的变性成分包括BSA发生反应。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser9 of human GSK-3β.

单克隆抗体是采用合成的与人类GSK-3β蛋白Ser9周围残基相对应的磷酸肽段免疫动物生产的。

IF-IC

IF-IC

Confocal immunofluorescent analysis of wild type mouse embryonic fibroblasts (MEFs) (top row), GSK-3β (-/-) MEFs (middle row) , or PC-3 cells (bottom row), untreated (left), LY294002- and Wortmannin-treated (#9901 and #9951 respectively; center) or lambda phosphatase-treated (right), using Phospho-GSK-3β (Ser9) (D85E12) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). (MEF wild type and GSK-3β (-/-) cells were kindly provided by Dr. Jim Woodgett, University of Toronto, Canada).

共聚焦免疫荧光分析野生型小鼠胚胎成纤维细胞 (MEFs) (top row), GSK-3β (-/-) MEFs (middle row) , or PC-3 cells (bottom row), 未处理组 (左), LY294002- 和 Wortmannin处理组 (#9901 and #9951 respectively; 中) or λ 磷酸酶处理 (右),所用抗体为Phospho-GSK-3β (Ser9) (D85E12) XP ® Rabbit mAb (绿色).蓝色伪彩是DRAQ5 ® #4084 (荧光 DNA 染料). ). (MEF wild type, GSK-3α (-/-) 和GSK-3β (-/-) 细胞是由加拿大多伦多大学Dr. Jim Woodgett友情提供).

Western Blotting

Western Blotting

Western blot analysis of extracts from GSK-3α (-/-) (lanes 1,2) and GSK-3β (-/-) (lanes 3,4) mouse embryonic fibroblast (MEF) cells, λ phosphatase or PDGF-treated, using Phospho-GSK-3β (Ser9) (D85E12) XP® Rabbit mAb (upper) and α/β-Tubulin Antibody #2148 (lower). (MEF wild type, GSK-3α (-/-) and GSK-3β (-/-) cells were kindly provided by Dr. Jim Woodgett, University of Toronto, Canada).

Western blot 分析GSK-3α (-/-) (泳道 1,2) 和 GSK-3β (-/-) (泳道 3,4)小鼠胚胎成纤维细胞提取物, λ磷酸酶或PDGF处理组,所用抗体为Phospho-GSK-3β (Ser9) (D85E12) XP ® Rabbit mAb兔单抗 (上) 和α/β-Tubulin Antibody #2148 (下). (MEF wild type, GSK-3α (-/-) 和GSK-3β (-/-) 细胞是由加拿大多伦多大学Dr. Jim Woodgett 友情提供).

Western Blotting

Western Blotting

Western blot analysis of extracts from PC-3 cells, untreated or LY294002/wortmannin-treated, using Phospho-GSK-3β (Ser9) (D85E12) XP® Rabbit mAb (upper) or GSK-3β (27C10) Rabbit mAb #9315 (lower).

Western blot 分析PC-3细胞提取物,未处理组和LY294002/wortmannin处理组,所用抗体为Phospho-GSK-3β (Ser9) (D85E12) XP ® Rabbit mAb 兔单抗(上) 或 GSK-3β (27C10) Rabbit mAb 兔单抗#9315 (下).

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of NIH/3T3 cells, untreated (blue) or PDGF-treated (green), using Phospho-GSK-3β (Ser9) (D85E12) XP® Rabbit mAb.

流式细胞术分析NIH/3T3 ,蓝色为未处理组,绿色为PDGF处理组,所用抗体为Phospho-GSK-3β (Ser9) (D85E12) XP® Rabbit mAb兔单抗。

Background

Glycogen synthase kinase-3 (GSK-3) was initially identified as an enzyme that regulates glycogen synthesis in response to insulin (1). GSK-3 is a ubiquitously expressed serine/threonine protein kinase that phosphorylates and inactivates glycogen synthase. GSK-3 is a critical downstream element of the PI3 kinase/Akt cell survival pathway whose activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3α and Ser9 of GSK-3β (2,3). GSK-3 has been implicated in the regulation of cell fate in Dictyostelium and is a component of the Wnt signaling pathway required for Drosophila, Xenopus, and mammalian development (4). GSK-3 has been shown to regulate cyclin D1 proteolysis and subcellular localization (5).

糖原合酶激酶-3(GSK-3)最初被确认为是一种能在胰岛素的刺激下参与调节糖原合成的酶(1)。GSK-3是一个广泛表达的丝氨酸/苏氨酸蛋白激酶,可以磷酸化并抑制糖原合成酶的活性。GSK-3是PI3K/ Akt细胞存活通路上的一个关键下游分子,其活性可以被Akt介导的GSK-3α的Ser21位点和GSK-3β的Ser9位点的磷酸化抑制(2,3)。GSK-3不仅参与盘基网柄菌细胞命运的调控,而且是果蝇,爪蟾和哺乳动物发育过程中所需的Wnt信号通路的一个组成部分(4)。GSK-3还参与调节cyclin D1 蛋白降解和亚细胞定位(5)。

  1. Welsh, G.I. et al. (1996) Trends Cell Biol 6, 274-9.
  2. Srivastava, A.K. and Pandey, S.K. (1998) Mol Cell Biochem 182, 135-41.
  3. Cross, D.A. et al. (1995) Nature 378, 785-9.
  4. Nusse, R. (1997) Cell 89, 321-3.
  5. Diehl, J.A. et al. (1998) Genes Dev 12, 3499-511.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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