Cell Signaling Technology

Product Pathways - Protein Stability

USP10 Antibody #5553

DUB   ubiquitin   USP  

No. Size Price
5553S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
5553 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 110 Rabbit
IP 1:50
IF-IC 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

USP10抗体识别内源性USP10的总蛋白。

Source / Purification

USP10多克隆抗体是通过合成与人USP10蛋白Leu163邻近氨基酸残基序列一致的肽段,免疫动物获得,抗体经Protein A和肽亲和层析纯化。 Western blot analysis of extracts from 293T cells, either mock transfected or transfected with a human USP10 construct, using USP10 Antibody. 使用USP10抗体对转染人USP10表达载体或转染对照载体的293T细胞提取物进行Western blot分析的结果。 Western blot analysis of extracts from various cell lines using USP10 Antibody. 使用USP10抗体对不同细胞裂解物进行Western blot分析的结果。 Confocal immunofluorescent analysis of HCT-116 cells using USP10 Antibody (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 使用USP10抗体对结肠癌HCT-116细胞进行共聚焦免疫荧光分析的结果(绿色),蓝色伪彩为 DRAQ5® #4084(DNA荧光染料)。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using USP10 Antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, either mock transfected or transfected with a human USP10 construct, using USP10 Antibody.

IF-IC

IF-IC

Confocal immunofluorescent analysis of HCT-116 cells using USP10 Antibody (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Background

泛素酶(UBEs)催化蛋白质泛素化,去泛素化酶(DUB)作用相反(1,2)。共有5个亚家族的去泛素化酶被发现,包括USP, UCH, OTU, MJD和JAMM酶。USP10含有与USP家族去泛素化酶代表性的半胱氨酸和组氨酸盒保守基序一致的氨基酸序列。 在翻译后水平,USP10通过蛋白与蛋白之间相互作用和磷酸化两种机制被调节。实际上,有研究发现USP10与G3BP(Ras-GAP SH3 domain binding protein)的相互作用抑制其催化泛素链解聚的能力(3)。而且,有研究发现ATM介导的USP10 Thr42和Ser337位点的磷酸化会提高其稳定性并促进其从胞浆转位到核内,参与在遗传毒性应激反应过程中p53的去泛素化、稳定性提高和激活(4)。USP10也在内体区室化中发挥作用,它能够介导CFTR的去泛素化,从而促进其在内吞过程的循环和在细胞表面的表达(5,6)。

  1. Nijman, S.M. et al. (2005) Cell 123, 773-86.
  2. Nalepa, G. et al. (2006) Nat Rev Drug Discov 5, 596-613.
  3. Soncini, C. et al. (2001) Oncogene 20, 3869-79.
  4. Yuan, J. et al. (2010) Cell 140, 384-96.
  5. Liu, J. et al. (2011) Cell 147, 223-34.
  6. Bomberger, J.M. et al. (2009) J Biol Chem 284, 18778-89.
  7. Bomberger, J.M. et al. (2011) Channels (Austin) 4, 150-4.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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